PMID- 16085541
OWN - NLM
STAT- MEDLINE
DCOM- 20051230
LR  - 20050808
IS  - 1024-5332 (Print)
IS  - 1024-5332 (Linking)
VI  - 10
IP  - 4
DP  - 2005 Aug
TI  - Donor hematopoietic cells from transgenic mice that express GFP are immunogenic 
      in immunocompetent recipients.
PG  - 289-95
AB  - OBJECTIVE: To study the immunogenicity of hematopoietic cells marked with green 
      fluorescence protein (GFP) while avoiding the potentially confounding effects of 
      viral gene transduction, marked cells from GFP+ transgenic mice were tracked 
      after transplantation into unconditioned immunocompetent recipients. MATERIALS 
      AND METHODS: Marrow was harvested from GFP+ transgenic mice that had been crossed 
      onto a BALB/cByJ background. Unconditioned marrow transplantation involved 
      infusion of sex-matched or sex-mismatched cells into female BALB/cByJ hosts. 
      Engraftment and contribution to circulating nucleated blood cells were compared 
      to recipients of donor cells that were not GFP-marked. Donor cells were detected 
      by flow cytometry (GFP) and fluorescence in situ hybridization (FISH) for 
      Y-chromosome sequences. RESULTS: Donor cells from mice of the same genetic 
      background that did not express GFP were detected for more than four-weeks in 
      unconditioned recipients. In contrast, GFP-marked cells in the blood peaked at 
      one-week, declined to undetectable levels by two-weeks and were not detected in 
      the marrow at sacrifice. In sex-mismatched studies, detection of male GFP+ donor 
      cells by FISH yielded levels similar to those observed by flow cytometry, in 
      contrast to the levels detected for many weeks in mice infused with male cells 
      that did not express GFP. In immunocompetent recipients immunized with irradiated 
      GFP-expressing cells, rechallenge with GFP+ cells resulted in the accelerated 
      loss of donor cells. CONCLUSION: Donor marrow cells from GFP+ transgenic mice 
      were lost after infusion into unconditioned immunocompetent mice and 
      sensitization studies infer an immunologic mechanism. These results are similar 
      to studies of virally transduced cells. Thus, infusion of cells with optimum 
      engraftment potential could not compensate for the loss of donor cells due to 
      immunogenicity.
FAU - Bubnic, Simon J
AU  - Bubnic SJ
AD  - Osiris Therapeutics Inc., 2001 Aliceanna Street, Baltimore, MD 21231, USA. 
      sbubnic@osiristx.com
FAU - Nagy, Andras
AU  - Nagy A
FAU - Keating, Armand
AU  - Keating A
LA  - eng
PT  - Journal Article
PL  - England
TA  - Hematology
JT  - Hematology (Amsterdam, Netherlands)
JID - 9708388
RN  - 147336-22-9 (Green Fluorescent Proteins)
SB  - IM
MH  - Animals
MH  - Blood Cells/*immunology
MH  - *Bone Marrow Transplantation
MH  - Female
MH  - Flow Cytometry
MH  - Graft Rejection/*immunology
MH  - Graft Survival/immunology
MH  - Green Fluorescent Proteins/genetics/*immunology
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Male
MH  - Mice
MH  - Mice, Transgenic
MH  - Transgenes/genetics/*immunology
MH  - Y Chromosome/genetics/immunology
EDAT- 2005/08/09 09:00
MHDA- 2005/12/31 09:00
CRDT- 2005/08/09 09:00
PHST- 2005/08/09 09:00 [pubmed]
PHST- 2005/12/31 09:00 [medline]
PHST- 2005/08/09 09:00 [entrez]
AID - H54731X1770018X3 [pii]
AID - 10.1080/10245330500093468 [doi]
PST - ppublish
SO  - Hematology. 2005 Aug;10(4):289-95. doi: 10.1080/10245330500093468.