PMID- 16091737
OWN - NLM
STAT- MEDLINE
DCOM- 20060112
LR  - 20220410
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 24
IP  - 56
DP  - 2005 Dec 15
TI  - Exclusion of RUNX3 as a tumour-suppressor gene in early-onset gastric carcinomas.
PG  - 8252-8
AB  - Recent studies claim a critical role for RUNX3 in gastric epithelial homeostasis. 
      However, conflicting results exist regarding RUNX3 expression in the stomach and 
      its potential role as a tumour-suppressor gene (TSG) in gastric carcinogenesis. 
      Our aim was to evaluate the role of RUNX3 in early-onset gastric carcinomas 
      (EOGCs). We analysed 41 EOGCs for RUNX3 aberrations using loss of heterozygosity 
      (LOH), fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) 
      analyses. LOH of markers flanking RUNX3 was relatively common, indicating that 
      loss of the gene may play a role in gastric carcinogenesis. However, FISH 
      analysis of selected cases and a panel of 14 gastric carcinoma-derived cell lines 
      showed widespread presence of multiple copies of centromere 1. While RUNX3 copy 
      numbers were generally equal to or fewer than those of centromere 1, at least two 
      copies were present in almost all cells analysed. Accordingly, a subpopulation of 
      tumour cells in 12/37 cases showed RUNX3 protein expression. However, expression 
      was not detected in the adjacent nontumorous mucosa of any case. Together, these 
      observations indicate that chromosome 1 aberrations occur frequently in EOGCs and 
      are reflected in the LOH and IHC patterns found. Our findings refute a role for 
      RUNX3 as a TSG in EOGCs.
CI  - Oncogene (2005) 24, 8252-8258. doi:10.1038/sj.onc.1208963; published online 1 
      August 2005.
FAU - Carvalho, Ralph
AU  - Carvalho R
AD  - Department of Pathology, Academisch Medisch Centrum, Amsterdam, The Netherlands. 
      r.p.carvalho@amc.uva.nl
FAU - Milne, Anya N A
AU  - Milne AN
FAU - Polak, Mirjam
AU  - Polak M
FAU - Corver, Willem E
AU  - Corver WE
FAU - Offerhaus, G Johan A
AU  - Offerhaus GJ
FAU - Weterman, Marian A J
AU  - Weterman MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Core Binding Factor Alpha 3 Subunit)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Runx3 protein, human)
RN  - 0 (Tumor Suppressor Proteins)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma/*genetics/pathology
MH  - Cell Line, Tumor
MH  - Core Binding Factor Alpha 3 Subunit/genetics/*physiology
MH  - *Genes, Tumor Suppressor
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Middle Aged
MH  - Neoplasm Proteins/genetics/physiology
MH  - Stomach Neoplasms/*genetics/pathology
MH  - Tumor Suppressor Proteins/genetics/physiology
EDAT- 2005/08/11 09:00
MHDA- 2006/01/13 09:00
CRDT- 2005/08/11 09:00
PHST- 2005/08/11 09:00 [pubmed]
PHST- 2006/01/13 09:00 [medline]
PHST- 2005/08/11 09:00 [entrez]
AID - 1208963 [pii]
AID - 10.1038/sj.onc.1208963 [doi]
PST - ppublish
SO  - Oncogene. 2005 Dec 15;24(56):8252-8. doi: 10.1038/sj.onc.1208963.