PMID- 16091891 OWN - NLM STAT- MEDLINE DCOM- 20051025 LR - 20131121 IS - 0141-5492 (Print) IS - 0141-5492 (Linking) VI - 27 IP - 13 DP - 2005 Jul TI - Autohydrogenotrophic denitrifying microbial community in a glass beads biofilm reactor. PG - 949-53 AB - A glass bead biofilm reactor was operated using H2 as an electron donor to remove nitrate at 150 mg NO3-N l(-1) to below detection level. The microbial community in the glass beads biofilm reactor was investigated by using denaturing gradient gel electrophoresis (DGGE) and phylogenetic analysis. In DGGE analysis of the biofilm, five bands were dominant and indicated the presence of eight beta-proteobacteria, one gamma-proteobacteria and twelve clostridia. An unculturable Hydrogenophaga sp., which is a new genus of hydrogen-oxidizing bacterium was dominant in microbial community of the biofilm reactor. FAU - Park, Ho Il AU - Park HI AD - Water Environment and Remediation Research Center, Korea Institute of Science and Technology, 136-791 Seoul, Korea. FAU - Choi, Yong-Jin AU - Choi YJ FAU - Pak, Daewon AU - Pak D LA - eng PT - Journal Article PL - Netherlands TA - Biotechnol Lett JT - Biotechnology letters JID - 8008051 RN - 0 (DNA, Bacterial) RN - 0 (Nitrates) RN - 0 (Nitrites) RN - 0 (RNA, Ribosomal, 16S) RN - 0 (Sewage) RN - 7YNJ3PO35Z (Hydrogen) SB - IM MH - Bacteria/genetics/growth & development/*metabolism MH - Betaproteobacteria/genetics/growth & development/metabolism MH - Biodiversity MH - *Biofilms MH - Bioreactors/*microbiology MH - Clostridium/genetics/growth & development/metabolism MH - Comamonadaceae/genetics/growth & development/metabolism MH - DNA, Bacterial/analysis/genetics MH - Electrophoresis, Polyacrylamide Gel/methods MH - Gammaproteobacteria/genetics/growth & development/metabolism MH - Glass MH - Hydrogen/metabolism MH - Microspheres MH - Nitrates/*metabolism MH - Nitrites/metabolism MH - Phylogeny MH - RNA, Ribosomal, 16S/genetics MH - Sewage/microbiology EDAT- 2005/08/11 09:00 MHDA- 2005/10/26 09:00 CRDT- 2005/08/11 09:00 PHST- 2004/11/22 00:00 [received] PHST- 2005/05/11 00:00 [accepted] PHST- 2005/08/11 09:00 [pubmed] PHST- 2005/10/26 09:00 [medline] PHST- 2005/08/11 09:00 [entrez] AID - 10.1007/s10529-005-7654-x [doi] PST - ppublish SO - Biotechnol Lett. 2005 Jul;27(13):949-53. doi: 10.1007/s10529-005-7654-x.