PMID- 16094515 OWN - NLM STAT- MEDLINE DCOM- 20051209 LR - 20181113 IS - 1434-7229 (Print) IS - 1434-7229 (Linking) VI - 8 IP - 2 DP - 2005 TI - Enhancement of liver-specific functions of primary rat hepatocytes co-cultured with bone marrow cells on tissue culture-treated polystyrene surfaces. PG - 104-9 AB - Primary rat hepatocytes were co-cultured with bone marrow cells (BMCs) in a tissue culture-treated culture well (TCW) or a collagen-coated culture well (CCW). Although a medium containing serum was used, the co-cultured cells formed spheroids on the TCW within 2 days, and ammonia metabolism and albumin secretion activities were well maintained for 3 weeks. The co-cultured cells formed a monolayer on the CCW within 2 days, and liver functions were maintained for 3 weeks. The ammonia metabolism activities and albumin secretion activities of co-cultured cells on the CCWs were slightly inferior to those on the TCWs. This co-culture system of hepatocytes and BMCs is an appropriate culture system for the expression and maintenance of liver-specific functions in vitro, and is expected to be applicable to bioartificial liver systems, regenerative medicine, and liver function simulators. FAU - Ijima, Hiroyuki AU - Ijima H AD - Department of Chemical Engineering, Kyushu University, Higashi-ku, Fukuoka 812-8581, Japan. ijima@chem-eng.kyushu-u.ac.jp FAU - Murakami, Sunao AU - Murakami S FAU - Matsuo, Takeshi AU - Matsuo T FAU - Takei, Takayuki AU - Takei T FAU - Ono, Tsutomu AU - Ono T FAU - Kawakami, Koei AU - Kawakami K LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Japan TA - J Artif Organs JT - Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs JID - 9815648 RN - 0 (Albumins) RN - 0 (Polystyrenes) RN - 7664-41-7 (Ammonia) SB - IM MH - Albumins/biosynthesis MH - Ammonia/metabolism MH - Animals MH - Bone Marrow Cells/cytology/*metabolism MH - *Coculture Techniques MH - Hepatocytes/*metabolism MH - Liver, Artificial MH - Male MH - *Polystyrenes MH - Rats MH - Rats, Wistar MH - Tissue Engineering/methods EDAT- 2005/08/12 09:00 MHDA- 2005/12/13 09:00 CRDT- 2005/08/12 09:00 PHST- 2003/11/18 00:00 [received] PHST- 2005/03/11 00:00 [accepted] PHST- 2005/08/12 09:00 [pubmed] PHST- 2005/12/13 09:00 [medline] PHST- 2005/08/12 09:00 [entrez] AID - 10.1007/s10047-005-0289-9 [doi] PST - ppublish SO - J Artif Organs. 2005;8(2):104-9. doi: 10.1007/s10047-005-0289-9.