PMID- 16094661
OWN - NLM
STAT- MEDLINE
DCOM- 20060421
LR  - 20151123
IS  - 0893-6692 (Print)
IS  - 0893-6692 (Linking)
VI  - 47
IP  - 1
DP  - 2006 Jan
TI  - 3,4-Epoxy-1-butene, a reactive metabolite of 1,3-butadiene, induces somatic 
      mutations in Xpc-null mice.
PG  - 67-70
AB  - Xpc-null (Xpc-/-) mice, deficient in the global genome repair subpathway of 
      nucleotide excision repair (NER-GGR), were exposed by intraperitoneal (i.p.) 
      injection to a 300 mg/kg mutagenic dose of 3,4-epoxy-1-butene (EB), to 
      investigate NER's potential role in repairing butadiene (BD) epoxide DNA lesions. 
      Mutagenic sensitivity was assessed using the Hprt assay. Xpc-/- mice were 
      significantly more sensitive to EB exposure, exhibiting an average 2.8-fold 
      increase in Hprt mutant frequency (MF) relative to those of exposed Xpc+/+ 
      (wild-type) mice. As a positive control for NER-GGR, additional mice were exposed 
      by i.p. injection to a 150 mg/kg mutagenic dose of benzo[a]pyrene (B[a]P). The 
      Xpc-/- mice had MFs 2.9-fold higher than those of exposed Xpc+/+ mice. These 
      results suggest that NER-GGR plays a role in recognizing and repairing some of 
      the DNA adducts formed following in vivo exposure to EB. Additional research is 
      needed to examine the response of Xpc-/- mice, as well as other NER-deficient 
      strains, to inhaled BD. Furthermore, it is likely that alternative DNA repair 
      pathways also are involved in restoring genomic integrity compromised by 
      BD-epoxide DNA damage. Collaborative studies are currently underway to address 
      these critical issues.
CI  - 2005 Wiley-Liss, Inc.
FAU - Wickliffe, J K
AU  - Wickliffe JK
AD  - Department of Preventive Medicine and Community Health, University of Texas 
      Medical Branch, Galveston, Texas, USA. jkwickli@utmb.edu
FAU - Galbert, L A
AU  - Galbert LA
FAU - Ammenheuser, M M
AU  - Ammenheuser MM
FAU - Herring, S M
AU  - Herring SM
FAU - Xie, J
AU  - Xie J
FAU - Masters, O E 3rd
AU  - Masters OE 3rd
FAU - Friedberg, E C
AU  - Friedberg EC
FAU - Lloyd, R S
AU  - Lloyd RS
FAU - Ward, J B Jr
AU  - Ward JB Jr
LA  - eng
GR  - 1 P30-ES06676/ES/NIEHS NIH HHS/United States
GR  - 2R01ES06015-07/ES/NIEHS NIH HHS/United States
GR  - S11 ES-10018/ES/NIEHS NIH HHS/United States
GR  - T32-ES07254/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (DNA Adducts)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Epoxy Compounds)
RN  - 0 (Mutagens)
RN  - 0 (Xpc protein, mouse)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 478ERR5NKR (3,4-epoxy-1-butene)
RN  - 9007-49-2 (DNA)
RN  - EC 2.4.2.8 (Hypoxanthine Phosphoribosyltransferase)
SB  - IM
MH  - Animals
MH  - Benzo(a)pyrene/toxicity
MH  - DNA/genetics
MH  - *DNA Adducts
MH  - DNA Repair
MH  - DNA-Binding Proteins/*deficiency/genetics
MH  - Epoxy Compounds/*toxicity
MH  - Genes, Reporter/genetics
MH  - Hypoxanthine Phosphoribosyltransferase/*genetics
MH  - Mice
MH  - Mice, Knockout
MH  - Mutagens/*toxicity
MH  - Mutation
EDAT- 2005/08/12 09:00
MHDA- 2006/04/25 09:00
CRDT- 2005/08/12 09:00
PHST- 2005/08/12 09:00 [pubmed]
PHST- 2006/04/25 09:00 [medline]
PHST- 2005/08/12 09:00 [entrez]
AID - 10.1002/em.20169 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 2006 Jan;47(1):67-70. doi: 10.1002/em.20169.