PMID- 16095007
OWN - NLM
STAT- MEDLINE
DCOM- 20050830
LR  - 20190911
IS  - 0300-9742 (Print)
IS  - 0300-9742 (Linking)
VI  - 34
IP  - 2
DP  - 2005 Mar-Apr
TI  - Anti-nucleosome antibodies in the diagnosis of systemic lupus erythematosus.
PG  - 122-4
AB  - OBJECTIVE: To study the prevalence and diagnostic significance of antibodies 
      against nucleosomes in patients with systemic lupus erythematosus (SLE) as 
      compared to five anti-nuclear antibody (ANA) assays. METHODS: The study included 
      305 patients with SLE, 125 patients with other autoimmune rheumatic diseases, and 
      415 healthy controls. Anti-nucleosome antibodies were measured by an 
      enzyme-linked immunosorbent assay (ELISA) and ANA by immunofluorescence (IF) 
      using Hep-2 cells. Anti-double-stranded DNA (anti-dsDNA) antibodies were measured 
      by three commercial ELISAs and by IF using Crithidia luciliae as antigen. 
      RESULTS: Compared to three ELISAs for anti-dsDNA, the anti-nucleosome assay was 
      less sensitive (30% vs. 29-69%) but equally specific (90% vs. 77-95%) for SLE. 
      The most sensitive test was ANA (76%), and the least sensitive was Crithidia 
      (13%). The correlations between the different assays were good (p < 0.001 for all 
      comparisons). CONCLUSION: The anti-nucleosome antibody assay does not offer 
      additional information compared to conventionally used anti-dsDNA tests in the 
      differential diagnosis of SLE.
FAU - Julkunen, H
AU  - Julkunen H
AD  - Department of Internal Medicine, Peijas Hospital, Vantaa, Finland. 
      heikki.julkunen@hus.fi
FAU - Salonen, E M
AU  - Salonen EM
FAU - Walle, T K
AU  - Walle TK
FAU - Miettinen, A
AU  - Miettinen A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand J Rheumatol
JT  - Scandinavian journal of rheumatology
JID - 0321213
RN  - 0 (Antibodies, Antinuclear)
RN  - 0 (Nucleosomes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Antibodies, Antinuclear/*blood
MH  - Arthritis, Rheumatoid/diagnosis/immunology
MH  - DNA/immunology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Humans
MH  - Lupus Erythematosus, Systemic/*diagnosis/*immunology
MH  - Male
MH  - Middle Aged
MH  - Nucleosomes/*immunology
MH  - Predictive Value of Tests
MH  - Reproducibility of Results
EDAT- 2005/08/13 09:00
MHDA- 2005/09/01 09:00
CRDT- 2005/08/13 09:00
PHST- 2005/08/13 09:00 [pubmed]
PHST- 2005/09/01 09:00 [medline]
PHST- 2005/08/13 09:00 [entrez]
AID - 10.1080/02813430510015250-1 [doi]
PST - ppublish
SO  - Scand J Rheumatol. 2005 Mar-Apr;34(2):122-4. doi: 10.1080/02813430510015250-1.