PMID- 16095529
OWN - NLM
STAT- MEDLINE
DCOM- 20060329
LR  - 20231105
IS  - 1465-993X (Electronic)
IS  - 1465-9921 (Print)
IS  - 1465-9921 (Linking)
VI  - 6
IP  - 1
DP  - 2005 Aug 11
TI  - A role for MCP-1/CCR2 in interstitial lung disease in children.
PG  - 93
AB  - BACKGROUND: Interstitial lung diseases (ILD) are chronic inflammatory disorders 
      leading to pulmonary fibrosis. Monocyte chemotactic protein 1 (MCP-1) promotes 
      collagen synthesis and deletion of the MCP-1 receptor CCR2 protects from 
      pulmonary fibrosis in ILD mouse models. We hypothesized that pulmonary MCP-1 and 
      CCR2+ T cells accumulate in pediatric ILD and are related to disease severity. 
      METHODS: Bronchoalveolar lavage fluid was obtained from 25 children with ILD and 
      10 healthy children. Levels of pulmonary MCP-1 and Th1/Th2-associated cytokines 
      were quantified at the protein and the mRNA levels. Pulmonary CCR2+, CCR4+, 
      CCR3+, CCR5+ and CXCR3+ T cells were quantified by flow-cytometry. RESULTS: CCR2+ 
      T cells and MCP-1 levels were significantly elevated in children with ILD and 
      correlated with forced vital capacity, total lung capacity and ILD disease 
      severity scores. Children with lung fibrosis had significantly higher MCP-1 
      levels and CCR2+ T cells in bronchoalveolar lavage fluid compared to non-fibrotic 
      children. CONCLUSION: The results indicate that pulmonary CCR2+ T cells and MCP-1 
      contribute to the pathogenesis of pediatric ILD and might provide a novel target 
      for therapeutic strategies.
FAU - Hartl, Dominik
AU  - Hartl D
AD  - Childrens' Hospital, Ludwig-Maximilians-University, Munich, Germany. 
      dominic.hartl@med.uni-muenchen.de
FAU - Griese, Matthias
AU  - Griese M
FAU - Nicolai, Thomas
AU  - Nicolai T
FAU - Zissel, Gernot
AU  - Zissel G
FAU - Prell, Christine
AU  - Prell C
FAU - Reinhardt, Dietrich
AU  - Reinhardt D
FAU - Schendel, Dolores J
AU  - Schendel DJ
FAU - Krauss-Etschmann, Susanne
AU  - Krauss-Etschmann S
LA  - eng
PT  - Controlled Clinical Trial
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050811
PL  - England
TA  - Respir Res
JT  - Respiratory research
JID - 101090633
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCR2 protein, human)
RN  - 0 (Ccr2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
SB  - IM
MH  - Adolescent
MH  - Asthma/*metabolism/pathology
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Chemokine CCL2/*metabolism
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Humans
MH  - Lung Diseases, Interstitial/*metabolism/pathology
MH  - Male
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/*metabolism
MH  - Severity of Illness Index
MH  - Th1 Cells/metabolism/pathology
MH  - Th2 Cells/metabolism/pathology
PMC - PMC1199626
EDAT- 2005/08/13 09:00
MHDA- 2006/03/30 09:00
PMCR- 2005/08/11
CRDT- 2005/08/13 09:00
PHST- 2005/04/19 00:00 [received]
PHST- 2005/08/11 00:00 [accepted]
PHST- 2005/08/13 09:00 [pubmed]
PHST- 2006/03/30 09:00 [medline]
PHST- 2005/08/13 09:00 [entrez]
PHST- 2005/08/11 00:00 [pmc-release]
AID - 1465-9921-6-93 [pii]
AID - 10.1186/1465-9921-6-93 [doi]
PST - epublish
SO  - Respir Res. 2005 Aug 11;6(1):93. doi: 10.1186/1465-9921-6-93.