PMID- 16095591
OWN - NLM
STAT- MEDLINE
DCOM- 20051114
LR  - 20161124
IS  - 0014-4827 (Print)
IS  - 0014-4827 (Linking)
VI  - 310
IP  - 1
DP  - 2005 Oct 15
TI  - A physiological model to study iron recycling in macrophages.
PG  - 43-53
AB  - Following erythrophagocytosis (EP) of senescent red blood cells (RBCs), heme iron 
      is recycled to the plasma by tissue macrophages. This process is critical for 
      mammalian iron homeostasis but remains elusive. We characterized a cellular model 
      using artificially-aged murine RBCs and murine bone marrow-derived macrophages 
      (BMDMs) and study mRNA and protein expression of HO-1, ferroportin and ferritin 
      after EP. In vitro ageing of RBCs was obtained by raising intracellular calcium 
      concentration. These RBCs exhibit several features of erythrocyte senescence 
      including externalization of phosphatidyl-serine, specific binding and 
      phagocytosis by BMDMs. During the first hours of EP, we observed a rapid increase 
      of HO-1 and ferroportin mRNAs and proteins, whereas ferritin protein expression 
      was progressively induced with no major changes in RNA levels. At later stages 
      after EP, a different pattern of expression was observed with a net decrease of 
      ferroportin, a sustained high level of HO-1, and a strong increase in ferritins. 
      Taken together, these results suggest that after EP, iron is rapidly extracted 
      from heme and exported by ferroportin. Surprisingly, the gene expression profile 
      at late stages after EP, which is indicative of iron storage, is reminiscent of 
      what is observed in inflammation. However, phagocytosis of artificially-aged red 
      blood cells seems to repress the proinflammatory response of macrophages.
FAU - Delaby, Constance
AU  - Delaby C
AD  - Inserm U656, Fer et synthese d'heme, Genetique, Physiologie et Pathologie, 
      Faculte de Medecine Xavier Bichat, 16, rue Henri Huchard, 75018 Paris, France.
FAU - Pilard, Nathalie
AU  - Pilard N
FAU - Hetet, Gilles
AU  - Hetet G
FAU - Driss, Fathi
AU  - Driss F
FAU - Grandchamp, Bernard
AU  - Grandchamp B
FAU - Beaumont, Carole
AU  - Beaumont C
FAU - Canonne-Hergaux, Francois
AU  - Canonne-Hergaux F
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Cation Transport Proteins)
RN  - 0 (Membrane Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (metal transporting protein 1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 9007-73-2 (Ferritins)
RN  - E1UOL152H7 (Iron)
RN  - EC 1.14.14.18 (Heme Oxygenase (Decyclizing))
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.14.14.18 (Hmox1 protein, mouse)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cation Transport Proteins/biosynthesis/genetics
MH  - Erythrocytes/metabolism/physiology
MH  - Ferritins/genetics/metabolism
MH  - Fluorescent Antibody Technique
MH  - Heme Oxygenase (Decyclizing)/biosynthesis/genetics
MH  - Heme Oxygenase-1
MH  - Inflammation
MH  - Iron/*metabolism
MH  - Macrophages/drug effects/metabolism/*physiology
MH  - Membrane Proteins
MH  - Mice
MH  - *Models, Biological
MH  - Nitric Oxide/metabolism
MH  - Phagocytosis/*physiology
MH  - RNA, Messenger/biosynthesis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2005/08/13 09:00
MHDA- 2005/11/15 09:00
CRDT- 2005/08/13 09:00
PHST- 2005/05/16 00:00 [received]
PHST- 2005/07/01 00:00 [revised]
PHST- 2005/07/05 00:00 [accepted]
PHST- 2005/08/13 09:00 [pubmed]
PHST- 2005/11/15 09:00 [medline]
PHST- 2005/08/13 09:00 [entrez]
AID - S0014-4827(05)00310-1 [pii]
AID - 10.1016/j.yexcr.2005.07.002 [doi]
PST - ppublish
SO  - Exp Cell Res. 2005 Oct 15;310(1):43-53. doi: 10.1016/j.yexcr.2005.07.002.