PMID- 16096949
OWN - NLM
STAT- MEDLINE
DCOM- 20051122
LR  - 20160526
IS  - 0743-684X (Print)
IS  - 0743-684X (Linking)
VI  - 21
IP  - 6
DP  - 2005 Aug
TI  - Early effect of gene therapy on a direct muscle neurotization model.
PG  - 383-9
AB  - Direct nerve-to-muscle neurotization has been the subject of both clinical and 
      experimental studies. In this study, the authors report a new animal model to 
      test the regenerative properties of a nerve (musculocutaneous) implanted in a 
      muscle (biceps). They also report the early effects of the application at the 
      implantation site of exogenously administered Brain Derived Nerve Factor (BDNF) 
      and of endogenously produced BDNF, via the administration of an adenoviral 
      construct with a tissue-specific promotor for muscle cells (AdRSV), and 
      containing the BDNF gene. Evaluation included behavioral testing (grooming test), 
      electrical stimulation, Western blot analysis of the distal implanted nerve to 
      determine the presence of locally produced BDNF, and motor end-plate staining of 
      the biceps muscle. At the early time point of 1 week following the 
      musculocutaneous nerve to biceps muscle implantation, there was no increased 
      production of recombinant BDNF at the distal implanted musculocutaneous nerve, as 
      assessed by Western blot analysis. Therefore, there was no significant difference 
      in the behavioral evaluation of the animals at 1 week; the Terzis grooming test 
      showed no statistical difference among groups, but a trend toward better function 
      for the BDNF and the high-dose AdRSV-BDNF groups, compared to the control groups. 
      There was also no difference in the histologic appearance and number of the motor 
      end-plates at the implantation site, compared to the controls. The electrical 
      stimulation of the MC nerve did not produce statistically significant results 
      among the experimental groups. In this direct nerve to muscle neurotization 
      model, the application of AdRSV-BDNF at 3 x 10 (9) pfu/ul did not show enhanced 
      production of BDNF at 1 week.
FAU - Papakonstantinou, Konstantinos C
AU  - Papakonstantinou KC
AD  - Microsurgery Research Center, Division of Plastic and Reconstructive Surgery, 
      Eastern Virginia Medical School, Norfolk, VA 23501, USA.
FAU - Terzis, Julia K
AU  - Terzis JK
FAU - Kamin, Elizabeth
AU  - Kamin E
FAU - Luka, Janos
AU  - Luka J
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Reconstr Microsurg
JT  - Journal of reconstructive microsurgery
JID - 8502670
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Growth Substances)
SB  - IM
MH  - Adenoviridae
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*administration & dosage
MH  - Genetic Therapy/*methods
MH  - Genetic Vectors
MH  - Growth Substances/*administration & dosage
MH  - Male
MH  - Models, Animal
MH  - Muscle, Skeletal
MH  - Nerve Regeneration/*drug effects
MH  - Nerve Transfer/*methods
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2005/08/13 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/13 09:00
PHST- 2005/08/13 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/13 09:00 [entrez]
AID - 10.1055/s-2005-915206 [doi]
PST - ppublish
SO  - J Reconstr Microsurg. 2005 Aug;21(6):383-9. doi: 10.1055/s-2005-915206.