PMID- 16097419
OWN - NLM
STAT- MEDLINE
DCOM- 20050908
LR  - 20191109
IS  - 1401-7431 (Print)
IS  - 1401-7431 (Linking)
VI  - 39
IP  - 1-2
DP  - 2005 Apr
TI  - Peripheral blood monocyte activation during coronary artery bypass grafting with 
      or without cardiopulmonary bypass.
PG  - 78-86
AB  - OBJECTIVE: The aim of this prospective, randomized study was to investigate the 
      impact of coronary artery bypass grafting (CABG) on peripheral monocytes and to 
      evaluate the additional effect of cardiopulmonary bypass (CPB). DESIGN: Twenty 
      patients admitted for elective CABG were randomized to either on-pump (ONCAB, n = 
      9) or off-pump (OFFCAB, n = 11) surgery and blood samples were drawn before, 
      during and 24 h after the operation. The total number of monocytes and the 
      proportion of the more mature CD16+/CD14+ monocytes were measured. Expression of 
      activation markers (CD11b, CD35 and CD62L) and oxidative burst were determined 
      using flow cytometry on both resting and in vitro stimulated cells. Serum 
      concentrations of soluble CD14 and monocytes/macrophage chemotactic protein 1 
      (MCP-1) were analysed. RESULTS: During surgery there was a selective decrease in 
      the proportion of CD16+/CD14+ monocytes compared to total monocytes. These had 
      returned to preoperative values 24 h after surgery while the total number of 
      monocytes had increased more than 100%. Intracellular production of oxygen free 
      radical H2O2 was increased in the ONCAB group during surgery compared to OFFCAB. 
      Monocyte expression and in vitro mobilization of complement receptors, CD11b and 
      CD35, were similar in both study groups during and after surgery as was the 
      expression of CD62L. Serum levels of MCP-1 decreased during surgery as did 
      soluble CD14, both with increased levels again the day after surgery. CONCLUSION: 
      It is concluded that the circulating monocyte population is activated during and 
      as a consequence of CABG. There were few apparent additional effects of CPB found 
      in this study. In this setting the inflammation caused by the surgery procedure 
      per se probably surpasses the impact of the CPB on circulating blood monocytes.
FAU - Wehlin, Lena
AU  - Wehlin L
AD  - Department of Clinical Immunology, Institution of Medicine, Karolinska 
      Institutet, Stockholm, Sweden. Lena.Wehlin@medks.ki.se
FAU - Vedin, Jenny
AU  - Vedin J
FAU - Vaage, Jarle
AU  - Vaage J
FAU - Lundahl, Joachim
AU  - Lundahl J
LA  - eng
PT  - Clinical Trial
PT  - Comparative Study
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Scand Cardiovasc J
JT  - Scandinavian cardiovascular journal : SCJ
JID - 9708377
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL4)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Macrophage Inflammatory Proteins)
SB  - IM
MH  - Aged
MH  - Biomarkers/blood
MH  - Cardiopulmonary Bypass/adverse effects/*methods
MH  - Chemokine CCL4
MH  - Coronary Angiography
MH  - Coronary Artery Bypass, Off-Pump/adverse effects/*methods
MH  - Coronary Stenosis/diagnostic imaging/mortality/*surgery
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Intraoperative Period
MH  - Lipopolysaccharide Receptors/*blood
MH  - Macrophage Inflammatory Proteins/*blood
MH  - Male
MH  - Middle Aged
MH  - Monocytes
MH  - Postoperative Care
MH  - Preoperative Care/methods
MH  - Probability
MH  - Prognosis
MH  - Prospective Studies
MH  - Risk Assessment
MH  - Sensitivity and Specificity
MH  - Severity of Illness Index
MH  - Survival Rate
MH  - Treatment Outcome
EDAT- 2005/08/16 09:00
MHDA- 2005/09/09 09:00
CRDT- 2005/08/16 09:00
PHST- 2005/08/16 09:00 [pubmed]
PHST- 2005/09/09 09:00 [medline]
PHST- 2005/08/16 09:00 [entrez]
AID - 10.1080/14017430410004623 [doi]
PST - ppublish
SO  - Scand Cardiovasc J. 2005 Apr;39(1-2):78-86. doi: 10.1080/14017430410004623.