PMID- 16098030
OWN - NLM
STAT- MEDLINE
DCOM- 20050913
LR  - 20131121
IS  - 0022-202X (Print)
IS  - 0022-202X (Linking)
VI  - 125
IP  - 2
DP  - 2005 Aug
TI  - H2O2 accumulation by catalase reduction changes MAP kinase signaling in aged 
      human skin in vivo.
PG  - 221-9
AB  - To understand the molecular alterations occurring during the aging process, we 
      compared mitogen-activated protein (MAP) kinase activities in the intrinsically 
      aged and photoaged skins in the same individuals. Furthermore, we investigated 
      the molecular events related to MAP kinase changes in intrinsically aged and 
      photoaged skins. We found that extracellular-signal-regulated kinase (ERK) 
      activity in photoaged skin was reduced, and that the activities of c-Jun 
      N-terminal kinase (JNK) and p38 kinase were increased compared with intrinsically 
      aged skin in the same individuals. Phospho-c-Jun levels and activator protein 1 
      activities in photoaged skin were also higher than in intrinsically aged skin. 
      Moreover, catalase activity was found to be much reduced in primary dermal 
      fibroblasts from photoaged skin, and as a result, H2O2 accumulated more in 
      primary dermal fibroblasts in photoaged skin. In addition, treating primary 
      dermal fibroblasts from photoaged skin with catalase reduced H2O2 levels, 
      reversed aging-dependent MAP kinase changes, and inhibited matrix 
      metalloproteinase (MMP)-1 expression. Our results indicate that the accumulation 
      of reactive oxygen species due to catalase attenuation may be a critical aspect 
      of the MAP kinase signaling changes that may lead to skin aging and photoaging in 
      human skin in vivo. Thus, the induction and regulation of endogenous antioxidant 
      enzymes including catalase may offer a strategy for preventing and treating skin 
      aging.
FAU - Shin, Mi Hee
AU  - Shin MH
AD  - Department of Dermatology, Laboratory of Cutaneous Agining Research, Clinical 
      Research Institute, Seoul National University College of Medicine, Seoul, Korea.
FAU - Rhie, Gi-eun
AU  - Rhie GE
FAU - Kim, Yeon Kyung
AU  - Kim YK
FAU - Park, Chi-Hyun
AU  - Park CH
FAU - Cho, Kwang Hyun
AU  - Cho KH
FAU - Kim, Kyu Han
AU  - Kim KH
FAU - Eun, Hee Chul
AU  - Eun HC
FAU - Chung, Jin Ho
AU  - Chung JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Invest Dermatol
JT  - The Journal of investigative dermatology
JID - 0426720
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (Transcription Factor AP-1)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Catalase/*metabolism/pharmacology
MH  - Cells, Cultured
MH  - Dermis/cytology/*enzymology
MH  - Extracellular Signal-Regulated MAP Kinases/metabolism
MH  - Female
MH  - Fibroblasts/cytology/enzymology
MH  - Humans
MH  - Hydrogen Peroxide/*metabolism
MH  - JNK Mitogen-Activated Protein Kinases/metabolism
MH  - MAP Kinase Signaling System/drug effects/*physiology
MH  - Male
MH  - Matrix Metalloproteinase 1/metabolism
MH  - Proto-Oncogene Proteins c-jun/metabolism
MH  - Skin Aging/*physiology
MH  - Transcription Factor AP-1/metabolism
MH  - Up-Regulation/physiology
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
EDAT- 2005/08/16 09:00
MHDA- 2005/09/15 09:00
CRDT- 2005/08/16 09:00
PHST- 2005/08/16 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/08/16 09:00 [entrez]
AID - S0022-202X(15)32396-4 [pii]
AID - 10.1111/j.0022-202X.2005.23823.x [doi]
PST - ppublish
SO  - J Invest Dermatol. 2005 Aug;125(2):221-9. doi: 10.1111/j.0022-202X.2005.23823.x.