PMID- 16100185
OWN - NLM
STAT- MEDLINE
DCOM- 20050906
LR  - 20061115
IS  - 0012-3692 (Print)
IS  - 0012-3692 (Linking)
VI  - 128
IP  - 2
DP  - 2005 Aug
TI  - Quantitative detection of lung cancer cells by fluorescence in situ 
      hybridization: comparison with conventional cytology.
PG  - 906-11
AB  - STUDY OBJECTIVE: The aim of this study was to clarify whether fluorescence in 
      situ hybridization (FISH) can diagnose lung cancer in various clinical specimens 
      in comparison with conventional cytology. DESIGN: Prospective study. SETTING: 
      University hospital in a metropolitan area. PATIENTS: Fifty consecutive patients 
      with abnormal chest radiography or CT scan findings were enrolled. The patients 
      included 32 men and 18 women, with an average age of 64 years. The final 
      definitive diagnosis was made by histologic examination, as follows: 38 primary 
      lung cancers (24 adenocarcinomas, 8 squamous cell carcinomas, 2 large cell 
      carcinomas, and 4 small cell carcinomas); 1 metastatic renal cell carcinoma; and 
      11 benign lesions. METHODS: Four types of clinical specimens were analyzed. Cells 
      obtained by transbronchial brushing and transbronchial fine-needle aspiration 
      using a fiberoptic bronchoscope under fluoroscopy, CT scan-guided percutaneous 
      needle biopsy, and bronchial washings. On every examination, duplicate slides 
      were made for analyses of conventional cytology and FISH. RESULTS: 
      Classifications according to conventional cytology were as follows: class I, 4 
      patients; class II, 15 patients; class IIIa, 3 patients; class IIIb, 5 patients; 
      and class V, 23 patients. A classification higher than class IIIb was considered 
      to be positive for cancer. For cytology, we found no false-positive cases and 11 
      false-negative cases. The specificity was 100%, and the sensitivity was 71.8%. By 
      FISH, 34 cases showed aberrant copy numbers in either chromosome 3 or 17. We 
      found no false-positive cases and five false-negative cases. The specificity was 
      100%, and the sensitivity was 87.1%. CONCLUSION: The ability of FISH to detect 
      aneusomic lung cancer cells is superior to conventional cytology for the 
      diagnosis of lung cancer.
FAU - Nakamura, Haruhiko
AU  - Nakamura H
AD  - Department of Chest Surgery, Atami Hospital, International University of Health 
      and Welfare, 13-1 Higashikaigan-cho, Atami-city, Shizuoka, Japan 413-0012. 
      h.nakamura@iuhw.ac.jp
FAU - Aute, Idiris
AU  - Aute I
FAU - Kawasaki, Norihito
AU  - Kawasaki N
FAU - Taguchi, Masahiko
AU  - Taguchi M
FAU - Ohira, Tatsuo
AU  - Ohira T
FAU - Kato, Harubumi
AU  - Kato H
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Chest
JT  - Chest
JID - 0231335
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lung Neoplasms/*pathology
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
EDAT- 2005/08/16 09:00
MHDA- 2005/09/07 09:00
CRDT- 2005/08/16 09:00
PHST- 2005/08/16 09:00 [pubmed]
PHST- 2005/09/07 09:00 [medline]
PHST- 2005/08/16 09:00 [entrez]
AID - S0012-3692(15)50443-1 [pii]
AID - 10.1378/chest.128.2.906 [doi]
PST - ppublish
SO  - Chest. 2005 Aug;128(2):906-11. doi: 10.1378/chest.128.2.906.