PMID- 16100341
OWN - NLM
STAT- MEDLINE
DCOM- 20051006
LR  - 20081120
IS  - 0003-4967 (Print)
IS  - 1468-2060 (Electronic)
IS  - 0003-4967 (Linking)
VI  - 64
IP  - 9
DP  - 2005 Sep
TI  - Potential relationship between herpes viruses and rheumatoid arthritis: analysis 
      with quantitative real time polymerase chain reaction.
PG  - 1357-9
AB  - OBJECTIVE: To determine whether the human herpes viruses, cytomegalovirus (CMV), 
      Epstein-Barr virus (EBV), and human herpesvirus 6 (HHV-6), are detectable in 
      serum and peripheral blood mononuclear cells (PBMCs) of patients with rheumatoid 
      arthritis (RA). METHODS: 133 PBMC samples (61 RA, 72 healthy donors) and 136 
      serum samples (59 RA, 77 healthy donors) were analysed by quantitative real time 
      polymerase chain reaction for DNA prevalence and viral load of HHV-6, EBV, and 
      CMV. RESULTS: For PBMC samples significant differences were found for EBV in DNA 
      prevalence (56% in RA v 33% in controls, p = 0.009) and viral load (copies/microg 
      DNA 0-592.3 for RA v 0-40.4 for controls, p = 0.001). For serum samples a 
      significant difference was found for HHV-6 DNA prevalence (10% in RA v 0% in 
      controls, p = 0.006) and viral load (copies/microg DNA 0-529.1 for RA v 0 for 
      controls, p = 0.007). CONCLUSIONS: Herpes viruses may have a role in RA, although 
      alternative explanations are possible: (a) defects in cellular immunity in 
      patients with RA may result in a relatively high viral load; (b) patients with RA 
      may be more prone to infection/reactivation. The usefulness of monitoring the DNA 
      viral load in patients with RA is questioned by these data.
FAU - Alvarez-Lafuente, R
AU  - Alvarez-Lafuente R
AD  - Service of Rheumatology, Hospital Clinico San Carlos, Profesor Martin, Lagos s/n, 
      28040 Madrid, Spain.
FAU - Fernandez-Gutierrez, B
AU  - Fernandez-Gutierrez B
FAU - de Miguel, S
AU  - de Miguel S
FAU - Jover, J A
AU  - Jover JA
FAU - Rollin, R
AU  - Rollin R
FAU - Loza, E
AU  - Loza E
FAU - Clemente, D
AU  - Clemente D
FAU - Lamas, J R
AU  - Lamas JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Ann Rheum Dis
JT  - Annals of the rheumatic diseases
JID - 0372355
RN  - 0 (Antirheumatic Agents)
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Aged, 80 and over
MH  - Antirheumatic Agents/therapeutic use
MH  - Arthritis, Rheumatoid/drug therapy/*virology
MH  - Cytomegalovirus/isolation & purification
MH  - DNA, Viral/analysis
MH  - Female
MH  - Herpesviridae/*isolation & purification
MH  - Herpesvirus 4, Human/isolation & purification
MH  - Herpesvirus 6, Human/isolation & purification
MH  - Humans
MH  - Leukocytes, Mononuclear/virology
MH  - Male
MH  - Middle Aged
MH  - Polymerase Chain Reaction/methods
MH  - Sex Factors
MH  - Viral Load
PMC - PMC1755640
EDAT- 2005/08/16 09:00
MHDA- 2005/10/07 09:00
PMCR- 2008/09/01
CRDT- 2005/08/16 09:00
PHST- 2005/08/16 09:00 [pubmed]
PHST- 2005/10/07 09:00 [medline]
PHST- 2005/08/16 09:00 [entrez]
PHST- 2008/09/01 00:00 [pmc-release]
AID - 64/9/1357 [pii]
AID - 10.1136/ard.2004.033514 [doi]
PST - ppublish
SO  - Ann Rheum Dis. 2005 Sep;64(9):1357-9. doi: 10.1136/ard.2004.033514.