PMID- 16101124
OWN - NLM
STAT- MEDLINE
DCOM- 20051013
LR  - 20071115
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 25
IP  - 5
DP  - 2005 Sep-Oct
TI  - Characterization of IGH rearrangements in non-Hodgkin's B-cell lymphomas by 
      fluorescence in situ hybridization.
PG  - 3179-82
AB  - Rearrangements involving the IGH gene have been identified in about 50% of 
      non-Hodgkin's B-cell lymphomas (NHL) and correlated to clinical relevant 
      subgroups. However, the detection rate varied greatly with the technique used. 
      The incidence of IGH rearrangements was analyzed using several fluorescence in 
      situ hybridization (FISH) techniques on metaphases obtained from 57 patients with 
      nodal NHL. An IGH rearrangement was identified in 42 cases (73.7%). A 
      t(14;18)(q32;q21) was found in 17 of the 20 follicular lymphomas (85%) studied 
      and a t(11;14)(q13;q32) in 10 of the 11 mantle cell lymphomas (91%). IGH 
      rearrangements were identified in 12 of the 26 diffuse large B-cell lymphomas 
      (46%), including 5 t(14;18)(q32;q21) and 2 t(3;14)(q27;q32). Conventional 
      cytogenetics was uninformative in several cases. However, the complemented 
      analysis using Multi-FISH and/or chromosomal whole paint enabled the 
      characterization of complex IGH translocations in follicular lymphomas and mantle 
      cell lymphomas and the identification of all the chromosomal partners involved in 
      the IGH rearrangement in diffuse large B-cell lymphomas. This study shows the 
      interest of using metaphase FISH in addition to conventional cytogenetics. 
      Following banding techniques, FISH with the IGH dual color probe could be the 
      first approach in NHL, after which chromosome painting and M-FISH could be used 
      to identify the chromosomal partner involved in the IGH rearrangement.
FAU - Bernicot, Izabel
AU  - Bernicot I
AD  - Laboratoire d'Histologie, Embryologie et Cytogenetique, Faculte de Medecine et 
      des Sciences de la Sante, Universite de Bretagne Occidentale, Brest, France.
FAU - Douet-Guilbert, Nathalie
AU  - Douet-Guilbert N
FAU - Le Bris, Marie-Josee
AU  - Le Bris MJ
FAU - Morice, Patrick
AU  - Morice P
FAU - Abgrall, Jean Francois
AU  - Abgrall JF
FAU - Berthou, Christian
AU  - Berthou C
FAU - Morel, Frederic
AU  - Morel F
FAU - De Braekeleer, Marc
AU  - De Braekeleer M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Immunoglobulin Heavy Chains)
SB  - IM
MH  - Chromosomes, Human, Pair 14/genetics
MH  - Chromosomes, Human, Pair 18/genetics
MH  - *Gene Rearrangement
MH  - Humans
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics/immunology
MH  - Lymphoma, Follicular/genetics/immunology
MH  - Lymphoma, Large B-Cell, Diffuse/genetics/immunology
MH  - Lymphoma, Mantle-Cell/genetics/immunology
MH  - Translocation, Genetic
EDAT- 2005/08/17 09:00
MHDA- 2005/10/14 09:00
CRDT- 2005/08/17 09:00
PHST- 2005/08/17 09:00 [pubmed]
PHST- 2005/10/14 09:00 [medline]
PHST- 2005/08/17 09:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 2005 Sep-Oct;25(5):3179-82.