PMID- 16101829
OWN - NLM
STAT- MEDLINE
DCOM- 20051228
LR  - 20210103
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 66
IP  - 3
DP  - 2005 Sep
TI  - Development and characterization of human constitutive proteasome and 
      immunoproteasome subunit-specific monoclonal antibodies.
PG  - 185-94
AB  - Delta (Y), MB1 (X), and Z are the three catalytic beta-subunits located in the 
      inner rings of the constitutive proteasome, an intracellular multicatalytic 
      complex responsible for the generation of peptides presented by human leukocyte 
      antigen (HLA) class I antigens to T cells. When cells are incubated with 
      interferon-gamma, delta (Y), MB1 (X), and Z are replaced by LMP2, LMP7, and 
      LMP10, respectively, leading to the expression of immunoproteasome which 
      generates peptides with increased affinity for HLA class I antigens. The 
      characterization of the expression of constitutive proteasome and 
      immunoproteasome subunits in cells, normal tissues, and malignant lesions has 
      been hampered by the lack or limited availability of constitutive proteasome and 
      immunoproteasome subunit-specific monoclonal antibodies (mAbs), which are 
      suitable for immunohistochemical staining. To overcome this limitation, we 
      generated human delta (Y), MB1 (X), Z, LMP2, LMP7, and LMP10-specific 
      mAb-secreting hybridomas from BALB/c mice immunized with peptides and recombinant 
      fusion proteins. The mAbs SY-5, SJJ-3, NB-1, SY-1, HB-2, and TO-7 were shown to 
      be specific for delta (Y), MB1 (X), Z, LMP2, LMP7, and LMP10, respectively, as 
      they react specifically with the corresponding molecules when tested with a human 
      B lymphoid LG2 cell lysate in Western blotting and with the peptide derived from 
      each molecule in enzyme-linked immunosorbent assay. The reactivity of the six 
      mAbs with the corresponding intracellular antigens resulted in intracellular 
      staining when the mAbs were tested with microwave-treated and 
      saponin-permeabilized cells in indirect immunofluorescence and with 
      formalin-fixed, paraffin-embedded tissue sections in immunohistochemical 
      reactions. These results suggest that the constitutive proteasome and 
      immunoproteasome subunit-specific mAbs we have developed are useful probes to 
      characterize the expression of proteasome subunits in normal tissues and in 
      pathological lesions.
FAU - Bandoh, N
AU  - Bandoh N
AD  - Department of Immunology, Roswell Park Cancer Institute, Buffalo, NY 14263, USA.
FAU - Ogino, T
AU  - Ogino T
FAU - Cho, H S
AU  - Cho HS
FAU - Hur, S Y
AU  - Hur SY
FAU - Shen, J
AU  - Shen J
FAU - Wang, X
AU  - Wang X
FAU - Kato, S
AU  - Kato S
FAU - Miyokawa, N
AU  - Miyokawa N
FAU - Harabuchi, Y
AU  - Harabuchi Y
FAU - Ferrone, S
AU  - Ferrone S
LA  - eng
GR  - R01 CA 67108/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (DNA Primers)
RN  - 0 (DNA, Complementary)
RN  - 0 (HLA Antigens)
RN  - 0 (Multienzyme Complexes)
RN  - 0 (Oligonucleotides)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 144416-78-4 (LMP-2 protein)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.25.1 (LMP7 protein)
RN  - EC 3.4.25.1 (PSMB10 protein, human)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal/*chemistry
MH  - Blotting, Western
MH  - Cell Line, Tumor
MH  - Cysteine Endopeptidases/immunology
MH  - DNA Primers/chemistry
MH  - DNA, Complementary/metabolism
MH  - Dose-Response Relationship, Immunologic
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique, Indirect
MH  - *Genes, MHC Class I
MH  - HLA Antigens/chemistry
MH  - Humans
MH  - Immunohistochemistry
MH  - *Major Histocompatibility Complex
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Fluorescence
MH  - Multienzyme Complexes/immunology
MH  - Oligonucleotides/chemistry
MH  - Peptides/chemistry
MH  - Proteasome Endopeptidase Complex/chemistry/*immunology/metabolism
MH  - Protein Binding
MH  - Recombinant Fusion Proteins/chemistry
MH  - T-Lymphocytes/metabolism
EDAT- 2005/08/17 09:00
MHDA- 2005/12/29 09:00
CRDT- 2005/08/17 09:00
PHST- 2005/08/17 09:00 [pubmed]
PHST- 2005/12/29 09:00 [medline]
PHST- 2005/08/17 09:00 [entrez]
AID - TAN462 [pii]
AID - 10.1111/j.1399-0039.2005.00462.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2005 Sep;66(3):185-94. doi: 10.1111/j.1399-0039.2005.00462.x.