PMID- 16103657
OWN - NLM
STAT- MEDLINE
DCOM- 20060123
LR  - 20080829
IS  - 1424-859X (Electronic)
IS  - 1424-8581 (Linking)
VI  - 111
IP  - 2
DP  - 2005
TI  - Karyotypic evolution and organization of the highly repetitive DNA sequences in 
      the Japanese shrew-moles, Dymecodon pilirostris and Urotrichus talpoides.
PG  - 152-8
AB  - The karyological relationship and organization of highly repetitive DNA sequences 
      in Japanese shrew-moles were studied by zoo-blot hybridization and fluorescence 
      in situ hybridization (FISH). When the genomic DNA of the eastern race of 
      Urotrichus talpoides was digested with PstI, three fragments of highly repetitive 
      DNA sequences, approximately 0.7, 0.9, and 1.4 kb in length, were observed as 
      distinct bands. The results of FISH in the eastern race of U. talpoides using 
      these three fragments separately as probes showed that the 0.7-kb PstI fragment 
      was distributed in the centromeric regions of most chromosomes, and that the 0.9- 
      and 1.4-kb fragments were predominantly located in the C-heterochromatin region 
      of chromosome 13p. Although the western race of U. talpoides also had three PstI 
      fragments, 0.9- and 1.4-kb PstI fragments were more ambiguous than those of the 
      eastern race. The PstI- digested genomic DNA in Dymecodonpilirostris produced 
      only a faint 0.9-kb band, and its signal patterns obtained by zoo-blot 
      hybridization were clearly different from those of U. talpoides. The 0.7-kb 
      fragment of U. talpoides hybridized strongly with the 0.9-kb fragment of D. 
      pilirostris. In a FISH analysis, the 0.9-kb fragment of D. pilirostris hybridized 
      with highly repetitive DNA in the centromeric regions of most chromosomes from 
      both D. pilirostris and U. talpoides. Zoo-blot hybridization and FISH analyses 
      suggest that the 0.9- and 1.4-kb PstI fragments were generated specifically in 
      the genome of U. talpoides after the common ancestor differentiated into two 
      extant shrew-mole species. A difference in the length of the centromeric elements 
      between U. talpoides and D. pilirostris might be observed due to certain 
      modifications of the repeating unit.
FAU - Nakata, A
AU  - Nakata A
AD  - Department of Biofunctional Science, Faculty of Agriculture and Life Science, 
      Hirosaki University, Hirosaki, Japan.
FAU - Yoshimura, A
AU  - Yoshimura A
FAU - Kuro-o, M
AU  - Kuro-o M
FAU - Obara, Y
AU  - Obara Y
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Genome Res
JT  - Cytogenetic and genome research
JID - 101142708
RN  - 9007-49-2 (DNA)
RN  - EC 3.1.21.4 (CTGCAG-specific type II deoxyribonucleases)
RN  - EC 3.1.21.4 (Deoxyribonucleases, Type II Site-Specific)
SB  - IM
MH  - Animals
MH  - Chromosome Mapping
MH  - DNA/*genetics
MH  - Deoxyribonucleases, Type II Site-Specific
MH  - *Evolution, Molecular
MH  - Geography
MH  - Japan
MH  - Karyotyping
MH  - Moles/*genetics
MH  - Repetitive Sequences, Nucleic Acid/*genetics
MH  - Restriction Mapping
MH  - Species Specificity
EDAT- 2005/08/17 09:00
MHDA- 2006/01/24 09:00
CRDT- 2005/08/17 09:00
PHST- 2004/11/05 00:00 [received]
PHST- 2005/02/18 00:00 [accepted]
PHST- 2005/08/17 09:00 [pubmed]
PHST- 2006/01/24 09:00 [medline]
PHST- 2005/08/17 09:00 [entrez]
AID - 86385 [pii]
AID - 10.1159/000086385 [doi]
PST - ppublish
SO  - Cytogenet Genome Res. 2005;111(2):152-8. doi: 10.1159/000086385.