PMID- 16104862
OWN - NLM
STAT- MEDLINE
DCOM- 20050923
LR  - 20131121
IS  - 1462-2912 (Print)
IS  - 1462-2912 (Linking)
VI  - 7
IP  - 9
DP  - 2005 Sep
TI  - In situ substrate conversion and assimilation by nitrifying bacteria in a model 
      biofilm.
PG  - 1392-404
AB  - Local nitrification and carbon assimilation activities were studied in situ in a 
      model biofilm to investigate carbon yields and contribution of distinct 
      populations to these activities. Immobilized microcolonies (related to 
      Nitrosomonas europaea/eutropha, Nitrosomonas oligotropha, Nitrospira sp., and to 
      other Bacteria) were incubated with [14C]-bicarbonate under different 
      experimental conditions. Nitrifying activity was measured concomitantly with 
      microsensors (oxygen, ammonium, nitrite, nitrate). Biofilm thin sections were 
      subjected to fluorescence in situ hybridization (FISH), microautoradiography 
      (MAR), and local quantification of [14C]-bicarbonate uptake (beta microimaging). 
      Nitrifying activity and tracer assimilation were restricted to a surface layer of 
      different thickness in the various experiments (substrate or oxygen limitation). 
      Excess oxygen uptake under all conditions revealed heterotrophic activity fuelled 
      by decay or excretion products during active nitrification. Depth limits and 
      intensity of tracer incorporation profiles were in agreement with 
      ammonia-oxidation activity (measured with microsensors), and distribution of 
      incorporated tracer (detected with MAR). Microautoradiography revealed a sharp 
      individual response of distinct populations in terms of in-/activity depending on 
      the (local) environmental conditions within the biofilm. Net in situ carbon 
      yields on N, expressed as e- equivalent ratios, varied between 0.005 and 0.018, 
      and, thus, were in the lower range of data reported for pure cultures of 
      nitrifiers.
FAU - Gieseke, Armin
AU  - Gieseke A
AD  - Microsensor Group, Max Planck Institute for Marine Microbiology, Celsiusstrasse 
      1, D-28359 Bremen, Germany. agieseke@mpi-bremen.de
FAU - Nielsen, Jeppe Lund
AU  - Nielsen JL
FAU - Amann, Rudolf
AU  - Amann R
FAU - Nielsen, Per Halkjaer
AU  - Nielsen PH
FAU - de Beer, Dirk
AU  - de Beer D
LA  - eng
PT  - Journal Article
PL  - England
TA  - Environ Microbiol
JT  - Environmental microbiology
JID - 100883692
RN  - 0 (Bicarbonates)
RN  - 0 (Carbon Radioisotopes)
RN  - 0 (Nitrates)
RN  - 0 (Nitrites)
RN  - 7664-41-7 (Ammonia)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Ammonia/*metabolism
MH  - Bicarbonates/metabolism
MH  - Biofilms/*growth & development
MH  - Carbon Radioisotopes
MH  - In Situ Hybridization, Fluorescence
MH  - Nitrates/*metabolism
MH  - Nitrites/*metabolism
MH  - Nitrosomonadaceae/*growth & development
MH  - Oxygen/metabolism
EDAT- 2005/08/18 09:00
MHDA- 2005/09/24 09:00
CRDT- 2005/08/18 09:00
PHST- 2005/08/18 09:00 [pubmed]
PHST- 2005/09/24 09:00 [medline]
PHST- 2005/08/18 09:00 [entrez]
AID - EMI826 [pii]
AID - 10.1111/j.1462-2920.2005.00826.x [doi]
PST - ppublish
SO  - Environ Microbiol. 2005 Sep;7(9):1392-404. doi: 10.1111/j.1462-2920.2005.00826.x.