PMID- 16104874
OWN - NLM
STAT- MEDLINE
DCOM- 20050926
LR  - 20061115
IS  - 0902-4441 (Print)
IS  - 0902-4441 (Linking)
VI  - 75
IP  - 3
DP  - 2005 Sep
TI  - Absence of p16 and p27 gene rearrangements and mutations in de novo 
      myelodysplastic syndromes.
PG  - 193-8
AB  - Myelodysplastic syndromes (MDS) represent a group of clonal hematopoietic 
      disorders characterized by dyshemopoiesis and frequent evolution to acute 
      leukemia. Tumor suppressor gene inactivation may be involved in MDS pathogenesis. 
      The two families of cyclin-dependent kinase inhibitors (CDKIs) (INK4 family of 
      p15, p16, p18 and p19 and CIP/KIP family of p21, p27 and p57) that negatively 
      regulate cell cycle progression are known tumor suppressor genes. To determine 
      whether genetic alterations of p16 and p27 genes play an important role in MDS 
      pathogenesis, we examined DNA from 51 patients classified as 17 refractory 
      anemias (RA), four refractory anemias with ringed sideroblasts (RARS), 19 
      refractory anemias with an excess of blasts (RAEB), 5 refractory anemias with 
      excess of blasts in transformation (RAEB-t) and 6 chronic myelomonocytic 
      leukemias (CMML). Southern blot analysis detected no homozygous deletions of p16 
      and p27. Polymerase chain reaction-single-strand conformation polymorphism 
      (PCR-SSCP) and sequencing did not reveal point mutations for both genes with the 
      exception of two allelic polymorphisms, namely a C --> G transition at 447 bp of 
      p16exon3 and a T --> A transition at 791 bp of p27exon1 genes. Our results 
      suggest that mutations of p16 and p27 genes resulting in abnormal p16 and p27 
      proteins do not represent a mechanism of gene inactivation involved in the 
      pathogenesis of MDS.
FAU - Papageorgiou, Sotirios G
AU  - Papageorgiou SG
AD  - Second Department of Internal Medicine Propaedeutic, Athens University Medical 
      School, Attikon General Hospital, Haidari, Greece. 
      sotirispapageorgeiou@hotmail.com
FAU - Pappa, Vassiliki
AU  - Pappa V
FAU - Papageorgiou, Efstathios
AU  - Papageorgiou E
FAU - Tsirigotis, Panagiotis
AU  - Tsirigotis P
FAU - Kontsioti, Frida
AU  - Kontsioti F
FAU - Panani, Anna
AU  - Panani A
FAU - Dervenoulas, John
AU  - Dervenoulas J
FAU - Economopoulos, Theofanis
AU  - Economopoulos T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Haematol
JT  - European journal of haematology
JID - 8703985
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Cyclin-Dependent Kinase Inhibitor p16)
RN  - 0 (DNA Primers)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 147604-94-2 (Cyclin-Dependent Kinase Inhibitor p27)
SB  - IM
MH  - Base Sequence
MH  - Blotting, Southern
MH  - Cell Cycle Proteins/*genetics
MH  - Cyclin-Dependent Kinase Inhibitor p16/*genetics
MH  - Cyclin-Dependent Kinase Inhibitor p27
MH  - DNA Primers
MH  - Humans
MH  - Karyotyping
MH  - *Mutation
MH  - Myelodysplastic Syndromes/*genetics
MH  - Polymerase Chain Reaction
MH  - Polymorphism, Single-Stranded Conformational
MH  - Tumor Suppressor Proteins/*genetics
EDAT- 2005/08/18 09:00
MHDA- 2005/09/27 09:00
CRDT- 2005/08/18 09:00
PHST- 2005/08/18 09:00 [pubmed]
PHST- 2005/09/27 09:00 [medline]
PHST- 2005/08/18 09:00 [entrez]
AID - EJH475 [pii]
AID - 10.1111/j.1600-0609.2005.00475.x [doi]
PST - ppublish
SO  - Eur J Haematol. 2005 Sep;75(3):193-8. doi: 10.1111/j.1600-0609.2005.00475.x.