PMID- 16105547
OWN - NLM
STAT- MEDLINE
DCOM- 20051011
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 335
IP  - 1
DP  - 2005 Sep 16
TI  - HHR23A, a human homolog of Saccharomyces cerevisiae Rad23, regulates xeroderma 
      pigmentosum C protein and is required for nucleotide excision repair.
PG  - 181-7
AB  - HHR23A and hHR23B are the human homologs of Saccharomyces cerevisiae Rad23. 
      hHR23B is associated with the nucleotide excision repair (NER) factor xeroderma 
      pigmentosum C (XPC) protein and is required for global genome repair. The 
      function of hHR23A is not yet clear. In this study, the potential function of the 
      hHR23A protein was investigated using RNA interference techniques. The hHR23A 
      knock-down (KD) construct diminished the RNA level of hHR23A protein by 
      approximately 60%, and it did not interfere with expression of the hHR23B gene. 
      Based on Southwestern immunoblot and host-cell reactivation assays, hHR23A(KD) 
      cells were found to be deficient in DNA repair activity against the DNA damage 
      caused by UVC irradiation. In these hHR23A(KD) cells, the XPC gene was not 
      normally induced by UVC irradiation, indicating that the hHR23A protein is 
      involved in NER through regulation of the DNA damage recognition protein XPC. 
      Co-immunoprecipitation experiments revealed that hHR23A was associated with a 
      small portion of hHR23B and the majority of p53 protein, indicating that hHR23A 
      regulates the function of XPC by its association with the NER activator p53.
FAU - Hsieh, Hui-Chuan
AU  - Hsieh HC
AD  - Medical Laboratory Science and Biotechnology, College of Medicine, National Cheng 
      Kung University, Tainan 70101, Taiwan.
FAU - Hsieh, Yi-Hsuan
AU  - Hsieh YH
FAU - Huang, Yu-Hsin
AU  - Huang YH
FAU - Shen, Fan-Ching
AU  - Shen FC
FAU - Tsai, Han-Ni
AU  - Tsai HN
FAU - Tsai, Jui-He
AU  - Tsai JH
FAU - Lai, Yu-Ting
AU  - Lai YT
FAU - Wang, Yu-Ting
AU  - Wang YT
FAU - Chuang, Woei-Jer
AU  - Chuang WJ
FAU - Huang, Wenya
AU  - Huang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (RAD23 protein, S cerevisiae)
RN  - 0 (RAD23B protein, human)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Tumor Suppressor Protein p53)
RN  - 156533-33-4 (RAD23A protein, human)
RN  - 156533-34-5 (XPC protein, human)
RN  - 9007-49-2 (DNA)
RN  - EC 6.5.1.- (DNA Repair Enzymes)
SB  - IM
MH  - Cell Line, Tumor
MH  - Cell Survival
MH  - DNA/*genetics
MH  - *DNA Repair
MH  - DNA Repair Enzymes
MH  - DNA-Binding Proteins/*chemistry/genetics/*metabolism
MH  - Gene Expression Regulation/genetics
MH  - Humans
MH  - Protein Binding
MH  - RNA Interference
MH  - Saccharomyces cerevisiae Proteins/*chemistry
MH  - Tumor Suppressor Protein p53/metabolism
EDAT- 2005/08/18 09:00
MHDA- 2005/10/12 09:00
CRDT- 2005/08/18 09:00
PHST- 2005/06/28 00:00 [received]
PHST- 2005/07/06 00:00 [accepted]
PHST- 2005/08/18 09:00 [pubmed]
PHST- 2005/10/12 09:00 [medline]
PHST- 2005/08/18 09:00 [entrez]
AID - S0006-291X(05)01456-7 [pii]
AID - 10.1016/j.bbrc.2005.07.067 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2005 Sep 16;335(1):181-7. doi: 
      10.1016/j.bbrc.2005.07.067.