PMID- 16105742
OWN - NLM
STAT- MEDLINE
DCOM- 20051129
LR  - 20211203
IS  - 1043-4666 (Print)
IS  - 1043-4666 (Linking)
VI  - 31
IP  - 6
DP  - 2005 Sep 21
TI  - MCP-1-stimulated chemotaxis of monocytic and endothelial cells is dependent on 
      activation of different signaling cascades.
PG  - 439-46
AB  - Monocyte chemoattractant protein-1 (MCP-1) is important in attracting monocytes 
      to sites of inflammation. Besides induction of monocyte recruitment, MCP-1 can 
      also affect chemotactic response of endothelial cells. The molecular mechanisms 
      involved in MCP-1-induced cell migration are poorly understood. In the current 
      investigation, we demonstrate activation of p42/44(ERK1/2) and p38 
      mitogen-activated protein kinases (MAPKs), phosphatydilinositol-3-kinase (PI3K) 
      and Src-kinases in both monocytes and endothelial cells stimulated with MCP-1 in 
      vitro. The response was rapid and time-dependent, detectable within 3 min of 
      MCP-1 stimulation. MCP-1-induced phosphorylation of p42/44(ERK1/2) MAPKs was 
      partially blocked by inhibitor of PI3K LY294002, while phosphorylation of p38 
      MAPK was diminished to a greater extent in presence of Src-kinase inhibitor PP2. 
      There was a substantial inhibition of monocyte migration upon treatment with 
      inhibitors of p38 MAPK, at the same time inhibition of p42/44(ERK1/2) MAPK 
      activation had no effect. On the contrary, the MCP-1-stimulated chemotaxis of 
      endothelial cells was completely abolished by inhibitors of PI3K and 
      p42/44(ERK1/2), but not by p38 MAPK inhibitors. These results suggest that 
      parallel signal transduction pathways are activated by MCP-1, and that depending 
      on the cell type these pathways differentially contribute to cell chemotactic 
      activity.
FAU - Arefieva, Tatiana I
AU  - Arefieva TI
AD  - Institute of Experimental Cardiology, Cardiology Research Centre, Moscow, Russian 
      Federation.
FAU - Kukhtina, Nadezhda B
AU  - Kukhtina NB
FAU - Antonova, Olga A
AU  - Antonova OA
FAU - Krasnikova, Tatiana L
AU  - Krasnikova TL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytokine
JT  - Cytokine
JID - 9005353
RN  - 0 (Chemokine CCL2)
RN  - EC 2.7.11.1 (Protein Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (Extracellular Signal-Regulated MAP Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Cells, Cultured
MH  - Chemokine CCL2/*pharmacology
MH  - Chemotaxis/*drug effects/physiology
MH  - Endothelial Cells/*drug effects/physiology
MH  - Extracellular Signal-Regulated MAP Kinases/drug effects/physiology
MH  - Fluorescent Antibody Technique
MH  - Humans
MH  - Monocytes/*drug effects/physiology
MH  - Phosphatidylinositol 3-Kinases/drug effects/physiology
MH  - Protein Serine-Threonine Kinases/drug effects/physiology
MH  - Signal Transduction/*drug effects
MH  - p38 Mitogen-Activated Protein Kinases/drug effects/physiology
EDAT- 2005/08/18 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/18 09:00
PHST- 2005/01/06 00:00 [received]
PHST- 2005/06/21 00:00 [revised]
PHST- 2005/06/29 00:00 [accepted]
PHST- 2005/08/18 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/18 09:00 [entrez]
AID - S1043-4666(05)00229-2 [pii]
AID - 10.1016/j.cyto.2005.06.016 [doi]
PST - ppublish
SO  - Cytokine. 2005 Sep 21;31(6):439-46. doi: 10.1016/j.cyto.2005.06.016.