PMID- 16106754
OWN - NLM
STAT- MEDLINE
DCOM- 20051004
LR  - 20190513
IS  - 1340-2838 (Print)
IS  - 1340-2838 (Linking)
VI  - 12
IP  - 1
DP  - 2005 Feb 28
TI  - Lotus burttii takes a position of the third corner in the lotus molecular 
      genetics triangle.
PG  - 69-77
AB  - In order to consolidate molecular genetic system in Lotus japonicus and to 
      further access the biological diversity in Lotea, we introduce here Lotus burttii 
      B-303 derived from West Pakistan as the third crossing partner of the Gifu 
      ecotype (B-129-S9) for a genetic analysis. L. burttii is a relatively small and 
      early flowering plant with non-shattering behavior. The general chromosome 
      morphology is very similar to Gifu, and fluorescence in situ hybridization (FISH) 
      analysis revealed that the short arm of chromosome 1 in L. burttii is comparable 
      to that of Gifu, indicating that the translocation event involving chromosomes 1 
      and 2, which was observed in L. japonicus Miyakojima MG-20, is not present in L. 
      burttii. In addition L. burttii has a higher level of DNA polymorphism compared 
      to Gifu and MG-20 enabling design of codominant markers such as SSR, CAPS and 
      dCAPS. Using an F2 population from a cross between Gifu and L. burttii, 
      codominant makers that co-segregated at the translocation site could be expanded. 
      In order to normalize the genetic background, L. burttii was inbred for nine 
      generations and the germplasm L. burttii B-303-S9 was established.
FAU - Kawaguchi, Masayoshi
AU  - Kawaguchi M
AD  - Department of Biological Sciences, Graduate School of Science, University of 
      Tokyo, 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, Japan. masayosi@biol.s.u-tokyo.ac.jp
FAU - Pedrosa-Harand, Andrea
AU  - Pedrosa-Harand A
FAU - Yano, Koji
AU  - Yano K
FAU - Hayashi, Makoto
AU  - Hayashi M
FAU - Murooka, Yoshikatsu
AU  - Murooka Y
FAU - Saito, Katsuharu
AU  - Saito K
FAU - Nagata, Toshiyuki
AU  - Nagata T
FAU - Namai, Kiyoshi
AU  - Namai K
FAU - Nishida, Hiroshi
AU  - Nishida H
FAU - Shibata, Daisuke
AU  - Shibata D
FAU - Sato, Shusei
AU  - Sato S
FAU - Tabata, Satoshi
AU  - Tabata S
FAU - Hayashi, Masaki
AU  - Hayashi M
FAU - Harada, Kyuya
AU  - Harada K
FAU - Sandal, Niels
AU  - Sandal N
FAU - Stougaard, Jens
AU  - Stougaard J
FAU - Bachmair, Andreas
AU  - Bachmair A
FAU - Grant, William F
AU  - Grant WF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - DNA Res
JT  - DNA research : an international journal for rapid publication of reports on genes 
      and genomes
JID - 9423827
RN  - 0 (DNA, Plant)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - Crosses, Genetic
MH  - DNA, Plant/genetics
MH  - Genes, Plant
MH  - Genetic Linkage
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Lotus/classification/*genetics
MH  - Molecular Biology
MH  - Molecular Sequence Data
MH  - Polymorphism, Genetic
MH  - Translocation, Genetic/genetics
EDAT- 2005/08/19 09:00
MHDA- 2005/10/05 09:00
CRDT- 2005/08/19 09:00
PHST- 2005/08/19 09:00 [pubmed]
PHST- 2005/10/05 09:00 [medline]
PHST- 2005/08/19 09:00 [entrez]
AID - 10.1093/dnares/12.1.69 [doi]
PST - ppublish
SO  - DNA Res. 2005 Feb 28;12(1):69-77. doi: 10.1093/dnares/12.1.69.