PMID- 16106910
OWN - NLM
STAT- MEDLINE
DCOM- 20051202
LR  - 20240109
IS  - 0171-9335 (Print)
IS  - 0171-9335 (Linking)
VI  - 84
IP  - 7
DP  - 2005 Jul
TI  - Multicellular structures developing during maize microspore culture express 
      endosperm and embryo-specific genes and show different embryogenic 
      potentialities.
PG  - 663-75
AB  - During maize pollen embryogenesis, a range of multicellular structures are 
      formed. Using different approaches, the "nature" of these structures has been 
      determined in terms of their embryogenic potential. In situ molecular 
      identification techniques for gene transcripts and products, and a novel cell 
      tracking system indicated the presence of embryogenic (embryo-like structures, 
      ELS) and non-embryogenic (callus-like structures, CLS) structures that occurred 
      for short periods within the cultures. Some multicellular structures with a 
      compact appearance generated embryos. RT-PCR and fluorescence in situ 
      hybridization (FISH) with confocal microscopy techniques using specific gene 
      markers of the endosperm (ZmESR2, ZmAE3) and embryo (LTP2 and ZmOCL1, ZmOCL3) 
      revealed "embryo" and "endosperm" potentialities in these various multicellular 
      structures present in the cultures. The results presented here showed distinct 
      and specific patterns of gene expression. Altogether, the results demonstrate the 
      presence of different molecules on both embryonic and non-embryonic structures. 
      Their possible roles are discussed in the context of a parallel between 
      embryo/endosperm interactions in planta and embryonic and non-embryonic structure 
      interrelations under in vitro conditions.
FAU - Massonneau, Agnes
AU  - Massonneau A
AD  - Reproduction et Developpement des Plantes, ENS Lyon, UMR5667, CNRS/INRA/ENS/LYON 
      1, 46 Allee d'Italie, F-69364 Lyon Cedex 07, France.
FAU - Coronado, Maria-Jose
AU  - Coronado MJ
FAU - Audran, Arthur
AU  - Audran A
FAU - Bagniewska, Agnieszka
AU  - Bagniewska A
FAU - Mol, Rafal
AU  - Mol R
FAU - Testillano, Pilar S
AU  - Testillano PS
FAU - Goralski, Grzegorz
AU  - Goralski G
FAU - Dumas, Christian
AU  - Dumas C
FAU - Risueno, Maria-Carmen
AU  - Risueno MC
FAU - Matthys-Rochon, Elisabeth
AU  - Matthys-Rochon E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Eur J Cell Biol
JT  - European journal of cell biology
JID - 7906240
RN  - 0 (Biomarkers)
RN  - 0 (Plant Proteins)
RN  - 0 (endosperm specific protein, Zea mays)
SB  - IM
MH  - Biomarkers
MH  - Embryonic Development
MH  - Gene Expression Regulation, Plant
MH  - Genes, Reporter/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Proteins/genetics
MH  - Pollen/cytology/embryology/genetics
MH  - Promoter Regions, Genetic/genetics
MH  - Seeds/genetics
MH  - Zea mays/*cytology/*embryology/genetics
EDAT- 2005/08/19 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/19 09:00
PHST- 2005/08/19 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/19 09:00 [entrez]
AID - S0171-9335(05)00062-2 [pii]
AID - 10.1016/j.ejcb.2005.02.002 [doi]
PST - ppublish
SO  - Eur J Cell Biol. 2005 Jul;84(7):663-75. doi: 10.1016/j.ejcb.2005.02.002.