PMID- 16108363
OWN - NLM
STAT- MEDLINE
DCOM- 20090723
LR  - 20181201
IS  - 1000-3061 (Print)
IS  - 1000-3061 (Linking)
VI  - 21
IP  - 3
DP  - 2005 May
TI  - [Flk1+ mesenchymal stem cells ameliorate carbon tetrachloride-induced liver 
      fibrosis in mice].
PG  - 396-401
AB  - Fibrosis is the common end stage of most liver diseases. Unfortunately, there is 
      no effective treatment available currently. This study was designed to evaluate 
      the effect of Flk1+ mesenchymal stem cells (MSC) from murine bone marrow (Flk1 + 
      MSC) on fibrosis formation induced by carbon tetrachloride (CCl4). In this study 
      Flk1+ MSC were isolated from bone marrow of male BALB/c mice. A CCl4 induced 
      hepatic fibrosis model was used. Flk1+ MSC were systemically infused immediately 
      or one week after the female mice were challenged with CCl4. Fibrosis index and 
      donor cell engraftment were assessed two or five weeks after CCl4 challenge. We 
      found that Flk1+ MSC transplantation immediately, but not one week after exposure 
      to CCl4, significantly reduced CCl4-induced liver damage and collagen deposition. 
      In addition, levels of hepatic hydroxyproline and serum fibrosis markers (HA, 
      P-III-P) in mice receiving immediate Flk1+ MSC transplantation after CCl4 
      challenge were significantly lower compared to those of control mice. More 
      importantly, histological examination suggested that hepatic damage recovery was 
      much better in these immediately Flk1+ MSC-treated mice. Immunofluorescence, PCR, 
      and fluorescence in situ hybridization (FISH) analysis revealed that donor cells 
      engrafted into host liver, had epithelium-like morphology and expressed albumin 
      (ALB), although at low frequency. In conclusion Flk1+ MSC might initiate 
      endogenous hepatic tissue regeneration, engraft into host liver in response to 
      CCl4 injury, and ameliorate its fibrogenic effects.
FAU - Shi, Ming-Xia
AU  - Shi MX
AD  - Tissue Engineering Center, Institute of Basic Medical Sciences, Chinese Academy 
      of Medical Sciences & Peking Union Medical College, Beijing 100005, China.
FAU - Fang, Bai-Jun
AU  - Fang BJ
FAU - Liao, Lian-Ming
AU  - Liao LM
FAU - Yang, Shao-Guang
AU  - Yang SG
FAU - Liu, Yu-Hao
AU  - Liu YH
FAU - Zhao, Chun-Hua
AU  - Zhao CH
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Wu Gong Cheng Xue Bao
JT  - Sheng wu gong cheng xue bao = Chinese journal of biotechnology
JID - 9426463
RN  - CL2T97X0V0 (Carbon Tetrachloride)
RN  - EC 2.7.10.1 (Vascular Endothelial Growth Factor Receptor-2)
SB  - IM
MH  - Animals
MH  - Carbon Tetrachloride
MH  - Female
MH  - Liver Cirrhosis, Experimental/*chemically induced/*therapy
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mesenchymal Stem Cells/cytology/metabolism/*physiology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Vascular Endothelial Growth Factor Receptor-2/metabolism
EDAT- 2005/08/20 09:00
MHDA- 2009/07/25 09:00
CRDT- 2005/08/20 09:00
PHST- 2005/08/20 09:00 [pubmed]
PHST- 2009/07/25 09:00 [medline]
PHST- 2005/08/20 09:00 [entrez]
PST - ppublish
SO  - Sheng Wu Gong Cheng Xue Bao. 2005 May;21(3):396-401.