PMID- 16110158
OWN - NLM
STAT- MEDLINE
DCOM- 20051206
LR  - 20060421
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 315
DP  - 2006
TI  - Techniques to study Fc epsilonRI signaling.
PG  - 175-89
AB  - Mast cells are the crucial effector cells for allergic reactions. They are 
      activated through the aggregation of the high-affinity immunoglobulin E (IgE) 
      receptor (Fc epsilonRI) with allergen and allergen-specific IgE. Tyrosine 
      phosphorylation of Fc epsilonRI subunits and various signaling proteins is an 
      initial triggering event, leading to the activation of several signaling pathways 
      in mast cells. Much has been learned from analysis of mast cells derived from 
      gene-targeted mice. Therefore, in this chapter we will first describe how to 
      generate mast cells from mouse bone marrow cells and how to correct the genetic 
      defect by retroviral transduction. Then, we will describe how to assess early 
      activation events by measuring several protein-tyrosine kinases and 
      serine/threonine kinases such as protein kinase B (= Akt), protein kinase C, and 
      JNK. As signal transduction is highly dependent on protein-protein interactions, 
      we will describe experimental details of co-immunoprecipitation methods that are 
      used to confirm such interactions.
FAU - Kawakami, Yuko
AU  - Kawakami Y
AD  - Division of Cell Biology, La Jolla Institute for Allergy and Immunology, San 
      Diego, CA, USA.
FAU - Kitaura, Jiro
AU  - Kitaura J
FAU - Kawakami, Toshiaki
AU  - Kawakami T
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Protein Subunits)
RN  - 0 (Receptors, IgE)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/physiology
MH  - Enzyme Activation
MH  - Genetic Vectors
MH  - Mast Cells/cytology/*physiology
MH  - Mice
MH  - Protein Subunits/*metabolism
MH  - Receptors, IgE/*metabolism
MH  - Retroviridae/genetics/metabolism
MH  - Signal Transduction/*physiology
EDAT- 2005/08/20 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/20 09:00
PHST- 2005/08/20 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/20 09:00 [entrez]
AID - 1-59259-967-2:175 [pii]
PST - ppublish
SO  - Methods Mol Biol. 2006;315:175-89.