PMID- 16110168
OWN - NLM
STAT- MEDLINE
DCOM- 20051206
LR  - 20190902
IS  - 1064-3745 (Print)
IS  - 1064-3745 (Linking)
VI  - 315
DP  - 2006
TI  - Mast cell activation by lipoproteins.
PG  - 341-8
AB  - Mast cells are activated by a number of agents that act independently from 
      immunoglobulin E (IgE)-mediated hypersensitivity. One of these agents is oxidized 
      low-density lipoprotein (oxLDL). OxLDL has been implicated in the pathogenesis of 
      atherosclerosis and has been shown to induce microvascular dysfunction by the 
      activation of mast cells. In this chapter, we describe the method for isolation 
      of human LDL, oxidation of LDL, and demonstrate that oxLDL activates mast cells 
      by measuring messenger ribonucleic acid (mRNA) levels and protein levels of 
      interleukin (IL)-8 an inflammatory cytokine. IL-8 is a potent chemoattractant for 
      neutrophils and monocytes, which would result in a chronic inflammatory response. 
      IL-8 mRNA levels were measured by reverse-transcription polymerase chain reaction 
      and protein levels by enzyme-linked immunoassay.
FAU - Kelley, Jim
AU  - Kelley J
AD  - Department of Internal Medicine, Quillen College of Medicine, East Tennessee 
      State University, Johnson City, TN, USA.
FAU - Hemontolor, Gregory
AU  - Hemontolor G
FAU - Younis, Walid
AU  - Younis W
FAU - Li, Chuanfu
AU  - Li C
FAU - Krishnaswamy, Guha
AU  - Krishnaswamy G
FAU - Chi, David S
AU  - Chi DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Culture Media)
RN  - 0 (Interleukin-8)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (oxidized low density lipoprotein)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Cell Line
MH  - Culture Media/chemistry
MH  - Culture Techniques
MH  - Humans
MH  - Immunoglobulin E/immunology
MH  - Interleukin-8/genetics/immunology
MH  - Lipoproteins, LDL/chemistry/*immunology
MH  - Mast Cells/cytology/*immunology
MH  - Oxidation-Reduction
EDAT- 2005/08/20 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/20 09:00
PHST- 2005/08/20 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/20 09:00 [entrez]
AID - 1-59259-967-2:341 [pii]
AID - 10.1385/1-59259-967-2:341 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2006;315:341-8. doi: 10.1385/1-59259-967-2:341.