PMID- 16110360
OWN - NLM
STAT- MEDLINE
DCOM- 20051107
LR  - 20220409
IS  - 1008-682X (Print)
IS  - 1008-682X (Linking)
VI  - 7
IP  - 3
DP  - 2005 Sep
TI  - Effects of tamoxifen citrate on gene expression during nuclear chromatin 
      condensation in male rats.
PG  - 311-21
AB  - AIM: To evaluate the effects of tamoxifen citrate on gene expression during 
      nuclear chromatin condensation in male rats. METHODS: The effects of an oral dose 
      of 0.4 kg/(kg.d) tamoxifen citrate on rates of in vitro chromatin decondensation, 
      acridine orange (AO) dye uptake, concentration of thiol-groups, levels and/or 
      expression of transition proteins 1, 2 (TP1, TP2), protamine 1 (P1), cyclic AMP 
      response element modulator-tau (CREMtau), androgen-binding protein (ABP) and 
      cyclic adenosine 3',5' monophosphate (cAMP) were evaluated after 60 days of 
      exposure in adult male rats. Controls received the vehicle. RESULTS: Tamoxifen 
      citrate enhanced the rates of chromatin decondensation, increased AO dye uptake 
      and reduced free thiols in caput epididymal sperms and reduced the levels of TP1, 
      TP2, P1, and CREMtau in the testis, while cAMP was unaffected. P1 deposition was 
      absent in the sperm. The transcripts of TP1, TP2 were increased, of P1 and ABP 
      decreased, while those of CREMtau unaffected in the testis. CONCLUSION: Tamoxifen 
      citrate reduced caput epididymal sperm chromatin compaction by reducing the 
      testicular levels of proteins TP1, TP2 and P1 and the CREMtau involved in 
      chromatin condensation during spermiogenesis. Tamoxifen citrate affects the 
      expression of these genes at both the transcriptional and post-transcriptional 
      levels.
FAU - Aleem, Mukhtar
AU  - Aleem M
AD  - Department of Neuroendocrinology, National Institute for Research in Reproductive 
      Health (ICMR), J. M. Street, Parel, Mumbai 400012, India.
FAU - Padwal, Varsha
AU  - Padwal V
FAU - Choudhari, Jyoti
AU  - Choudhari J
FAU - Balasinor, Nafisa
AU  - Balasinor N
FAU - Parte, Priyanka
AU  - Parte P
FAU - Gill-Sharma, Manjeet
AU  - Gill-Sharma M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Asian J Androl
JT  - Asian journal of andrology
JID - 100942132
RN  - 0 (Chromatin)
RN  - 0 (DNA Primers)
RN  - 0 (Sulfhydryl Compounds)
RN  - 094ZI81Y45 (Tamoxifen)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Western
MH  - Cell Nucleus/*drug effects/metabolism
MH  - Chromatin/*metabolism
MH  - Cyclic AMP/metabolism
MH  - DNA Primers
MH  - Gene Expression/*drug effects
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Spermatogenesis
MH  - Spermatozoa/*drug effects/metabolism/ultrastructure
MH  - Sulfhydryl Compounds/metabolism
MH  - Tamoxifen/*pharmacology
MH  - Testis/metabolism/ultrastructure
EDAT- 2005/08/20 09:00
MHDA- 2005/11/08 09:00
CRDT- 2005/08/20 09:00
PHST- 2005/08/20 09:00 [pubmed]
PHST- 2005/11/08 09:00 [medline]
PHST- 2005/08/20 09:00 [entrez]
AID - 10.1111/j.1745-7262.2005.00027.x [doi]
PST - ppublish
SO  - Asian J Androl. 2005 Sep;7(3):311-21. doi: 10.1111/j.1745-7262.2005.00027.x.