PMID- 16110436
OWN - NLM
STAT- MEDLINE
DCOM- 20060410
LR  - 20070502
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 4
IP  - 2
DP  - 2005 Jun 30
TI  - Uses and limitations of two molecular cytogenetic techniques for the study of 
      arrested embryos obtained through assisted reproduction technology.
PG  - 143-51
AB  - We studied chromosomal abnormalities in arrested embryos produced by assisted 
      reproductive technology with fluorescence in situ hybridization (FISH) and 
      comparative genomic hybridization (CGH) in order to determine the best technique 
      for evaluating chromosomal aneusomies to be implemented in different situations. 
      We examined individual blastomeres from arrested embryos by FISH and arrested 
      whole embryos by CGH. All of the 10 FISH-analyzed embryos gave results, while 
      only 7 of the 30 embryos analyzed by CGH were usable. Fifteen of the 17 embryos 
      were chromosomally abnormal. CGH provided more accurate data for arrested 
      embryos; however, FISH is the technique of choice for screening in 
      preimplantation genetic diagnosis, because the results can be obtained within a 
      day, while the embryos are still in culture.
FAU - Muhlmann, Maria C
AU  - Muhlmann MC
AD  - Laboratorio de Citogenetica Molecular, Radiobiologia, CAC-CONICET, Argentina. 
      muhlmann@cnea.gov.ar
FAU - Laudicina, Alejandro O
AU  - Laudicina AO
FAU - Perandones, Claudia
AU  - Perandones C
FAU - Bertolino, Maria V
AU  - Bertolino MV
FAU - Marazzi, Andrea
AU  - Marazzi A
FAU - Quintans, Carlos J
AU  - Quintans CJ
FAU - Donaldson, Monica
AU  - Donaldson M
FAU - Bozzo, Walter
AU  - Bozzo W
FAU - Pasqualini, Sergio
AU  - Pasqualini S
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050630
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
SB  - IM
MH  - Chromosome Disorders/*diagnosis/embryology/genetics
MH  - Female
MH  - *Genomics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping/*methods
MH  - Pregnancy
MH  - Preimplantation Diagnosis/*methods
MH  - Reproductive Techniques, Assisted
EDAT- 2005/08/20 09:00
MHDA- 2006/04/11 09:00
CRDT- 2005/08/20 09:00
PHST- 2005/08/20 09:00 [pubmed]
PHST- 2006/04/11 09:00 [medline]
PHST- 2005/08/20 09:00 [entrez]
AID - 0126 [pii]
PST - epublish
SO  - Genet Mol Res. 2005 Jun 30;4(2):143-51.