PMID- 16112167
OWN - NLM
STAT- MEDLINE
DCOM- 20051223
LR  - 20050921
IS  - 0043-1354 (Print)
IS  - 0043-1354 (Linking)
VI  - 39
IP  - 16
DP  - 2005 Oct
TI  - Rapid quantification of bacteria in activated sludge using fluorescence in situ 
      hybridization and epifluorescence microscopy.
PG  - 3837-48
AB  - A rapid quantification method for bacteria in activated sludge has been 
      developed, based on fluorescence in situ hybridization (FISH) and epifluorescence 
      microscopy. Samples are hybridized on slides and analyzed by direct microscopic 
      observation. Abundance categories were designed based on digital images of the 
      target organisms. These rating systems were developed for the filamentous 
      bacteria Microthrix parvicella and for different morphotypes of nocardioform 
      actinomycetes, but can easily be adapted to other types of microorganisms. Due to 
      the quantification by direct microscopic observation, this method is suitable for 
      samples that are difficult to be processed by semi-automated image analysis 
      techniques, such as samples containing fluorescent debris, cells of different 
      fluorescence intensities and target organisms that need partial enzymatic 
      digestion prior to FISH. In contrast to commonly used rating systems consisting 
      of photographs, the newly developed categories allow to quantitatively compare 
      results of different categories and different organisms. The uncertainties of the 
      results were calculated by a non-parametric bootstrap procedure; a thorough 
      uncertainty analysis was performed including sample variability and operator 
      subjectivity.
FAU - Hug, Thomas
AU  - Hug T
AD  - EAWAG, Swiss Federal Institute for Aquatic Science and Technology, Ueberlandstr. 
      133, PO Box 611, CH-8600 Duebendorf, Switzerland.
FAU - Gujer, Willi
AU  - Gujer W
FAU - Siegrist, Hansruedi
AU  - Siegrist H
LA  - eng
PT  - Journal Article
PL  - England
TA  - Water Res
JT  - Water research
JID - 0105072
RN  - 0 (DNA, Bacterial)
SB  - IM
MH  - *Bacteria
MH  - DNA, Bacterial/*analysis
MH  - Environmental Monitoring/methods
MH  - In Situ Hybridization, Fluorescence
MH  - Microscopy, Fluorescence
MH  - Reproducibility of Results
MH  - Water Microbiology
EDAT- 2005/08/23 09:00
MHDA- 2005/12/24 09:00
CRDT- 2005/08/23 09:00
PHST- 2004/09/14 00:00 [received]
PHST- 2005/07/05 00:00 [revised]
PHST- 2005/07/08 00:00 [accepted]
PHST- 2005/08/23 09:00 [pubmed]
PHST- 2005/12/24 09:00 [medline]
PHST- 2005/08/23 09:00 [entrez]
AID - S0043-1354(05)00398-2 [pii]
AID - 10.1016/j.watres.2005.07.013 [doi]
PST - ppublish
SO  - Water Res. 2005 Oct;39(16):3837-48. doi: 10.1016/j.watres.2005.07.013.