PMID- 16113212
OWN - NLM
STAT- MEDLINE
DCOM- 20060123
LR  - 20240314
IS  - 0032-0889 (Print)
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Linking)
VI  - 139
IP  - 1
DP  - 2005 Sep
TI  - Chlorophyll breakdown in senescent Arabidopsis leaves. Characterization of 
      chlorophyll catabolites and of chlorophyll catabolic enzymes involved in the 
      degreening reaction.
PG  - 52-63
AB  - During senescence, chlorophyll (chl) is metabolized to colorless nonfluorescent 
      chl catabolites (NCCs). A central reaction of the breakdown pathway is the ring 
      cleavage of pheophorbide (pheide) a to a primary fluorescent chl catabolite. Two 
      enzymes catalyze this reaction, pheide a oxygenase (PAO) and red chl catabolite 
      reductase. Five NCCs and three fluorescent chl catabolites (FCCs) accumulated 
      during dark-induced chl breakdown in Arabidopsis (Arabidopsis thaliana). Three of 
      these NCCs and one FCC (primary fluorescent chl catabolite-1) were identical to 
      known catabolites from canola (Brassica napus). The presence in Arabidopsis of 
      two modified FCCs supports the hypothesis that modifications, as present in NCCs, 
      occur at the level of FCC. Chl degradation in Arabidopsis correlated with the 
      accumulation of FCCs and NCCs, as well as with an increase in PAO activity. This 
      increase was due to an up-regulation of Pao gene expression. In contrast, red chl 
      catabolite reductase is not regulated during leaf development and senescence. A 
      pao1 knockout mutant was identified and analyzed. The mutant showed an age- and 
      light-dependent cell death phenotype on leaves and in flowers caused by the 
      accumulation of photoreactive pheide a. In the dark, pao1 exhibited a stay-green 
      phenotype. The key role of PAO in chl breakdown is discussed.
FAU - Pruzinska, Adriana
AU  - Pruzinska A
AD  - Institute of Plant Sciences, University of Bern, Switzerland.
FAU - Tanner, Gaby
AU  - Tanner G
FAU - Aubry, Sylvain
AU  - Aubry S
FAU - Anders, Iwona
AU  - Anders I
FAU - Moser, Simone
AU  - Moser S
FAU - Muller, Thomas
AU  - Muller T
FAU - Ongania, Karl-Hans
AU  - Ongania KH
FAU - Krautler, Bernhard
AU  - Krautler B
FAU - Youn, Ji-Young
AU  - Youn JY
FAU - Liljegren, Sarah J
AU  - Liljegren SJ
FAU - Hortensteiner, Stefan
AU  - Hortensteiner S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050819
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (T-DNA)
RN  - 1406-65-1 (Chlorophyll)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.13.- (Oxygenases)
RN  - EC 1.13.- (pheide a oxygenase)
RN  - EC 1.3.7.- (ACD2 protein, Arabidopsis)
SB  - IM
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Arabidopsis/*enzymology/metabolism
MH  - Arabidopsis Proteins/metabolism
MH  - Cell Death
MH  - Chlorophyll/*analogs & derivatives/chemistry/*metabolism
MH  - DNA, Bacterial/genetics
MH  - Gene Expression Regulation, Enzymologic
MH  - Gene Expression Regulation, Plant
MH  - Light
MH  - Molecular Structure
MH  - Mutagenesis, Insertional/genetics
MH  - Oxidoreductases/metabolism
MH  - Oxygenases/genetics/metabolism
MH  - Phenotype
MH  - Plant Leaves/enzymology/*growth & development/*metabolism
MH  - Time Factors
PMC - PMC1203357
EDAT- 2005/08/23 09:00
MHDA- 2006/01/24 09:00
PMCR- 2006/09/01
CRDT- 2005/08/23 09:00
PHST- 2005/08/23 09:00 [pubmed]
PHST- 2006/01/24 09:00 [medline]
PHST- 2005/08/23 09:00 [entrez]
PHST- 2006/09/01 00:00 [pmc-release]
AID - pp.105.065870 [pii]
AID - 065870 [pii]
AID - 10.1104/pp.105.065870 [doi]
PST - ppublish
SO  - Plant Physiol. 2005 Sep;139(1):52-63. doi: 10.1104/pp.105.065870. Epub 2005 Aug 
      19.