PMID- 16115022
OWN - NLM
STAT- MEDLINE
DCOM- 20060104
LR  - 20131121
IS  - 1523-0864 (Print)
IS  - 1523-0864 (Linking)
VI  - 7
IP  - 9-10
DP  - 2005 Sep-Oct
TI  - Requirements for the different cysteines in the chemotactic and desensitizing 
      activity of human thioredoxin.
PG  - 1189-94
AB  - Thioredoxin (Trx) is a protein disulfide oxidoreductase that can be secreted and 
      act as a chemoattractant for leukocytes. Like chemokines, it causes 
      desensitization of monocytes against its chemotactic activity and that of 
      monocyte chemoattractant protein-1 (MCP-1). To investigate the role of the redox 
      properties of Trx, and particularly of some of its five cysteines, in its 
      chemotactic and desensitizing action, we tested different mutants, including 
      Trx80, a truncated form, and various mutants lacking specific cysteines: Trx 
      C62S/C73S and the redox-inactive mutant Trx C32S/C35S. Of the mutants, only Trx80 
      maintained the chemotactic activity of wild-type Trx toward both monocytes and 
      polymorphonuclear neutrophils, all of them desensitized monocytes against 
      wild-type Trx or MCP-1, but not chemotactic peptide formyl-methionyl-leucil 
      peptide. These data indicate that different redox-active cysteines are important 
      for Trx chemotactic action, whereas its desensitizing action does not have these 
      requirements, suggesting a redox-independent mechanism.
FAU - Bizzarri, Cinzia
AU  - Bizzarri C
AD  - Dompe' Research Center and Consorzio Biolaq, L'Aquila, Italy.
FAU - Holmgren, Arne
AU  - Holmgren A
FAU - Pekkari, Klas
AU  - Pekkari K
FAU - Chang, Geng
AU  - Chang G
FAU - Colotta, Francesco
AU  - Colotta F
FAU - Ghezzi, Pietro
AU  - Ghezzi P
FAU - Bertini, Riccardo
AU  - Bertini R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Antioxid Redox Signal
JT  - Antioxidants & redox signaling
JID - 100888899
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 0 (Peptides)
RN  - 0 (Recombinant Proteins)
RN  - 52500-60-4 (Thioredoxins)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - K848JZ4886 (Cysteine)
SB  - IM
MH  - Cell Movement
MH  - Chemokine CCL2/metabolism
MH  - Chemokines/metabolism
MH  - *Chemotaxis
MH  - Cysteine/chemistry/metabolism/*physiology
MH  - Dose-Response Relationship, Drug
MH  - Humans
MH  - Leukocytes, Mononuclear/cytology
MH  - Monocytes/cytology/metabolism
MH  - *Mutation
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/metabolism
MH  - Oxidation-Reduction
MH  - Peptides/chemistry
MH  - Recombinant Proteins/chemistry
MH  - Thioredoxins/*chemistry/genetics/metabolism
EDAT- 2005/08/24 09:00
MHDA- 2006/01/05 09:00
CRDT- 2005/08/24 09:00
PHST- 2005/08/24 09:00 [pubmed]
PHST- 2006/01/05 09:00 [medline]
PHST- 2005/08/24 09:00 [entrez]
AID - 10.1089/ars.2005.7.1189 [doi]
PST - ppublish
SO  - Antioxid Redox Signal. 2005 Sep-Oct;7(9-10):1189-94. doi: 
      10.1089/ars.2005.7.1189.