PMID- 16115368
OWN - NLM
STAT- MEDLINE
DCOM- 20061031
LR  - 20231213
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 26
IP  - 9
DP  - 2005 Sep
TI  - Involvement of nociceptin/orphanin FQ in release of hypothalamic GnRH mediated by 
      ORL1 receptor in ovariectomized rats.
PG  - 1039-44
AB  - AIM: To investigate effect of the nociceptin/orphanin FQ (OFQ) on hypothalamus 
      gonadotropin-releasing hormone (GnRH) release in ovariectomized (OVX) rats. 
      METHODS: GnRH radioimmunoassay (RIA) was used to study the effect of OFQ on GnRH 
      release in hypothalamus slices in vitro. Push-pull perfusion and 
      intracerebroventicular (icv) injection were used to examine the effect of OFQ on 
      GnRH release in the hypothalamus medial preoptic area (POA) in vivo. 
      Ovariectomies were performed on female Sprague-Dawley rats, and their plasma 
      luteinizing hormone (LH) levels were measured after icv injection of OFQ with or 
      without [Nphe1]NC(1-13)NH2, a competitive antagonist of opioid receptor-like1 
      receptor (ORL1 receptor). Reverse transcription-polymerase chain reaction 
      (RT-PCR) was used to investigate the expression of the ORL1 receptor in rat 
      pituitary. RESULTS: GnRH release from hypothalamus slices was inhibited 90 min 
      after the administration of 2 mmol/L and 20 mmol/L OFQ (P<0.05). Accordingly, 
      GnRH release from hypothalamus POA was also significantly reduced by the 
      injection of 0.2 mmol/L and 2 mmol/L OFQ. Plasma LH levels were also decreased 
      significantly 2 h after icv injection of 20 nmol OFQ in OVX rats (P<0.05) and 
      this effect could be abolished by pretreatment with 20 nmol [Nphe1]NC(1-13)NH2, 
      that is, NC13. More interestingly, plasma LH levels in OVX rats increased 
      markedly 2 h after icv injection of 100 nmol and 200 nmol NC13. RT-PCR analysis 
      further revealed that the ORL1 receptor was not expressed in the pituitary of OVX 
      rats. CONCLUSION: Central administration of nociceptin/orphanin FQ might inhibit 
      the release of hypothalamic GnRH and decrease the plasma LH levels through ORL1 
      receptors in OVX rats.
FAU - An, Xiao-fei
AU  - An XF
AD  - Department of Neurobiology and Integrative Medicine, Shanghai Medical 
      College,Fudan University, Shanghai 200032, China.
FAU - Chen, Hai-ping
AU  - Chen HP
FAU - Ma, Shu-lan
AU  - Ma SL
FAU - Feng, Yi
AU  - Feng Y
FAU - Hao, Jun-wei
AU  - Hao JW
FAU - Chen, Bo-ying
AU  - Chen BY
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (Opioid Peptides)
RN  - 0 (Receptors, Opioid)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 9002-67-9 (Luteinizing Hormone)
RN  - 0 (Nociceptin Receptor)
RN  - 0 (Oprl protein, rat)
SB  - IM
MH  - Animals
MH  - Female
MH  - Gonadotropin-Releasing Hormone/*metabolism
MH  - Hypothalamus/*metabolism
MH  - Luteinizing Hormone/blood
MH  - Opioid Peptides/administration & dosage/*pharmacology
MH  - Ovariectomy
MH  - Pituitary Gland/*metabolism
MH  - Preoptic Area/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Opioid/*metabolism
MH  - Nociceptin Receptor
MH  - Nociceptin
EDAT- 2005/08/24 09:00
MHDA- 2006/11/01 09:00
CRDT- 2005/08/24 09:00
PHST- 2005/08/24 09:00 [pubmed]
PHST- 2006/11/01 09:00 [medline]
PHST- 2005/08/24 09:00 [entrez]
AID - 10.1111/j.1745-7254.2005.00172.x [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2005 Sep;26(9):1039-44. doi: 
      10.1111/j.1745-7254.2005.00172.x.