PMID- 16115380
OWN - NLM
STAT- MEDLINE
DCOM- 20061031
LR  - 20081121
IS  - 1671-4083 (Print)
IS  - 1671-4083 (Linking)
VI  - 26
IP  - 9
DP  - 2005 Sep
TI  - Effects of CpG oligodeoxynucleotide on transcription factors GATA-3 and T-bet 
      mRNA expression in asthmatic mice.
PG  - 1117-22
AB  - AIM: To investigate effects of CpG oligodeoxynucleotide (CpG ODN) on the mRNA 
      expression of transcription factors GATA binding protein 3 (GATA-3) and T-box 
      expressed in T cells (T-bet) in asthmatic mice. METHODS: An asthmatic mouse model 
      was established and treated with CpG ODN. Total inflammatory cells and 
      eosinophils in bronchoalveolar lavage fluid (BALF) were counted and inflammatory 
      cell infiltration in lung tissue was evaluated. Interferon-gamma and 
      interleukin-4 concentrations in BALF and splenocyte culture supernatants were 
      detected using an enzyme-linked immunosorbent assay. Transcription factor GATA-3 
      and T-bet mRNA expression in splenocytes and lung tissue were detected by reverse 
      transcription-polymerase chain reaction. RESULTS: Total inflammatory cells and 
      eosinophils in BALF were reduced in the CpG ODN-treated group compared with the 
      asthma group, and inflammatory cell infiltration in lung tissue was also 
      significantly alleviated. CpG ODN treatment increased the interferon-gamma 
      concentration but decreased the interleukin-4 concentration in both BALF and 
      splenocyte culture supernatants. GATA-3 mRNA expression was reduced in both lung 
      tissue and splenocytes in the CpG ODN-treated group, while the mRNA ratio of 
      T-bet to GATA-3 in splenocytes was increased. CONCLUSION: CpG ODN treatment 
      inhibits airway inflammatory cell infiltration and regulates 
      interferon-gamma/interleukin-4 synthesis in asthmatic mice, possibly through a 
      mechanism of downregulation of GATA-3 mRNA expression in both lung tissue and 
      splenocytes.
FAU - Wu, Zu-qun
AU  - Wu ZQ
AD  - Department of Respiratory Medicine, the Second Affiliated Hospital, 4Department 
      of Pathology, the First Affiliated Hospital, School of Medicine, Zhejiang 
      University, Hangzhou 310009, China. wuzuqun522@sohu.com
FAU - Xu, Yi-ping
AU  - Xu YP
FAU - Xiang, Hua
AU  - Xiang H
FAU - Shen, Hua-hao
AU  - Shen HH
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Acta Pharmacol Sin
JT  - Acta pharmacologica Sinica
JID - 100956087
RN  - 0 (CPG-oligonucleotide)
RN  - 0 (GATA3 Transcription Factor)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (RNA, Messenger)
RN  - 0 (T-Box Domain Proteins)
RN  - 0 (T-box transcription factor TBX21)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Animals
MH  - Asthma/*metabolism/pathology
MH  - Bronchoalveolar Lavage Fluid/chemistry/cytology
MH  - Eosinophils/cytology/metabolism
MH  - GATA3 Transcription Factor/*biosynthesis/genetics
MH  - Interferon-gamma/metabolism
MH  - Interleukin-4/metabolism
MH  - Lung/metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oligodeoxyribonucleotides/*pharmacology
MH  - RNA, Messenger/biosynthesis
MH  - Spleen/cytology/metabolism
MH  - T-Box Domain Proteins/*biosynthesis/genetics
MH  - T-Lymphocytes/metabolism
EDAT- 2005/08/24 09:00
MHDA- 2006/11/01 09:00
CRDT- 2005/08/24 09:00
PHST- 2005/08/24 09:00 [pubmed]
PHST- 2006/11/01 09:00 [medline]
PHST- 2005/08/24 09:00 [entrez]
AID - 10.1111/j.1745-7254.2005.00157.x [doi]
PST - ppublish
SO  - Acta Pharmacol Sin. 2005 Sep;26(9):1117-22. doi: 
      10.1111/j.1745-7254.2005.00157.x.