PMID- 16115543
OWN - NLM
STAT- MEDLINE
DCOM- 20051102
LR  - 20131121
IS  - 0955-2863 (Print)
IS  - 0955-2863 (Linking)
VI  - 16
IP  - 9
DP  - 2005 Sep
TI  - Protective effect of polyphenol-containing azuki bean (Vigna angularis) seed 
      coats on the renal cortex in streptozotocin-induced diabetic rats.
PG  - 547-53
AB  - This study was undertaken to investigate the effect of azuki bean (Vigna 
      angularis) seed coats (ABSC), which contain polyphenols, on the infiltration of 
      macrophages and the progression of diabetic nephropathy in streptozotocin 
      (STZ)-induced diabetic rats. The diabetic rats were divided into three groups 
      with 0% (commercial diet), 0.1% and 1.0% ABSC diets. The vehicle-injected 
      controls were given a commercial diet. At 10 weeks, the macrophage kinetics, the 
      degree of fibrosis in glomeruli and mRNA expression for monocyte chemoattractant 
      protein-1 (MCP-1) were examined. There was no difference in plasma glucose levels 
      between diabetic rats treated with and without ABSC. The plasma levels of 
      malondialdehyde (MDA) in the ABSC-treated diabetic rats were significantly lower 
      than those in the untreated diabetic rats. Histopathologically, the percentage of 
      the fibrotic areas stained by Sirius red stain in the glomeruli in the 
      ABSC-treated diabetic rats was lower than in the untreated diabetic rats. 
      ED1-positive macrophages in the glomeruli and tubulointerstitium in the untreated 
      diabetic rats showed a significant increase in number compared with the controls. 
      In contrast, the number of macrophages in the ABSC-treated diabetic rats was 
      smaller than that in untreated diabetic rats. MCP-1 mRNA expression, estimated by 
      real-time quantitative RT-PCR, was increased 2.5-fold in the untreated diabetic 
      rat kidney, while a lower level was observed in the ABSC-treated diabetic rats. 
      In conclusion, our results suggest that ABSC treatments suppress the increased 
      number of infiltrating macrophages and MCP-1 mRNA expression, and attenuated the 
      glomerular expansion in STZ-induced rat diabetic nephropathy.
FAU - Sato, Shin
AU  - Sato S
AD  - Department of Life Sciences, Graduate School of Health Sciences, Aomori 
      University of Health and Welfare, Aomori 030-8505, Japan. s_sato3@auhw.ac.jp
FAU - Yamate, Jyoji
AU  - Yamate J
FAU - Hori, Yuuka
AU  - Hori Y
FAU - Hatai, Asako
AU  - Hatai A
FAU - Nozawa, Megumi
AU  - Nozawa M
FAU - Sagai, Masaru
AU  - Sagai M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Blood Glucose)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Flavonoids)
RN  - 0 (Phenols)
RN  - 0 (Polyphenols)
RN  - 0 (Protective Agents)
RN  - 0 (RNA, Messenger)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 5W494URQ81 (Streptozocin)
SB  - IM
MH  - Animals
MH  - Blood Glucose/metabolism
MH  - Chemokine CCL2/drug effects/genetics
MH  - Diabetes Mellitus, Experimental/complications/*drug therapy
MH  - Diabetic Nephropathies/drug therapy/etiology/*pathology
MH  - Fabaceae/*chemistry
MH  - Flavonoids/pharmacology
MH  - Kidney Cortex/*drug effects/metabolism/pathology
MH  - Macrophages/drug effects/pathology
MH  - Male
MH  - Malondialdehyde/metabolism
MH  - Phenols/pharmacology
MH  - Polyphenols
MH  - Protective Agents/*pharmacology
MH  - RNA, Messenger/drug effects
MH  - Rats
MH  - Rats, Wistar
MH  - Seeds/chemistry
MH  - Streptozocin
EDAT- 2005/08/24 09:00
MHDA- 2005/11/03 09:00
CRDT- 2005/08/24 09:00
PHST- 2005/01/12 00:00 [received]
PHST- 2005/02/02 00:00 [revised]
PHST- 2005/02/02 00:00 [accepted]
PHST- 2005/08/24 09:00 [pubmed]
PHST- 2005/11/03 09:00 [medline]
PHST- 2005/08/24 09:00 [entrez]
AID - S0955-2863(05)00038-0 [pii]
AID - 10.1016/j.jnutbio.2005.02.003 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2005 Sep;16(9):547-53. doi: 10.1016/j.jnutbio.2005.02.003.