PMID- 16116953
OWN - NLM
STAT- MEDLINE
DCOM- 20060530
LR  - 20240203
IS  - 1672-0733 (Print)
IS  - 1672-0733 (Linking)
VI  - 25
IP  - 2
DP  - 2005
TI  - Dual effect of 3,4-dihydroxyacetophenone on LPS-induced apoptosis in RAW264.7 
      cells by modulating the production of TNF-alpha.
PG  - 131-4
AB  - To explore the pharmacological effect of 3,4-dihydroxyacetophenone (DHAP) on the 
      apoptosis of RAW264.7 macrophage cells and the mechanism, RAW264.7 macrophage 
      cells were treated with 100 or 500 mg/L lipopolysaccharide (LPS), with or without 
      10(-5) mol/L DHAP for 24 h. Trypan blue dye exclusion assay was used to assess 
      cell viability. Cell apoptosis was morphological studied and flow cytometric 
      assay was used. Tumor necrosis factor-alpha (TNF-alpha) level was measured by 
      ELISA methods. IkappaB protein was determined by Western blotting. Our results 
      showed that in 100 mg/L LPS-stimulated macrophages, DHAP enhanced the cell 
      apoptosis while in 500 mg/L LPS-stimulated macrophages, DHAP significantly 
      inhibited the cell apoptosis. In both groups, DHAP increased the level of IkappaB 
      but decreased the level of TNF-alpha. It is concluded that DHAP has dual effect 
      on the apoptosis of RAW 264.7 cells treated with different concentrations of LPS. 
      This effect may be due to the inhibition of activation of NF-kappaB and autocrine 
      production of TNFalpha. Our study suggests that DHAP may have anti-inflammatory 
      effect on LPS-activated macrophages.
FAU - Wu, Ping
AU  - Wu P
AD  - Department of Pathophysiology, Tongji Medical College, Huazhong Science and 
      Technology University, Wuhan 430030, China.
FAU - Ye, Duyun
AU  - Ye D
FAU - Zhang, Daijuan
AU  - Zhang D
FAU - Zhang, Li
AU  - Zhang L
FAU - Wan, Jingyuan
AU  - Wan J
FAU - Pan, Qian
AU  - Pan Q
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - J Huazhong Univ Sci Technolog Med Sci
JT  - Journal of Huazhong University of Science and Technology. Medical sciences = Hua 
      zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. 
      Yixue Yingdewen ban
JID - 101169627
RN  - 0 (Acetophenones)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 07OQ35LVBK (3,4-dihydroxyacetophenone)
SB  - IM
MH  - Acetophenones/*pharmacology
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line
MH  - Lipopolysaccharides/*pharmacology
MH  - Macrophages/cytology/*metabolism
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Tumor Necrosis Factor-alpha/*biosynthesis
EDAT- 2005/08/25 09:00
MHDA- 2006/05/31 09:00
CRDT- 2005/08/25 09:00
PHST- 2005/08/25 09:00 [pubmed]
PHST- 2006/05/31 09:00 [medline]
PHST- 2005/08/25 09:00 [entrez]
AID - 10.1007/BF02873557 [doi]
PST - ppublish
SO  - J Huazhong Univ Sci Technolog Med Sci. 2005;25(2):131-4. doi: 10.1007/BF02873557.