PMID- 16116990
OWN - NLM
STAT- MEDLINE
DCOM- 20060517
LR  - 20191109
IS  - 1615-9306 (Print)
IS  - 1615-9306 (Linking)
VI  - 28
IP  - 11
DP  - 2005 Jul
TI  - Determination of enrofloxacin and its metabolite ciprofloxacin by high 
      performance capillary electrophoresis with end-column amperometric detection.
PG  - 1143-8
AB  - Enrofloxacin (ENR) and its metabolite ciprofloxacin (CIP) were determined by 
      capillary zone electrophoresis (CZE) with end-column amperometric detection. The 
      effect of several factors, such as pH and concentration of running buffer 
      solution, separation voltage, injection time, and working potential, on CZE were 
      investigated to establish the optimal conditions of separation and detection. 
      Under a given set of conditions (pH 8.00 phosphate buffer solution (20 mmol/L); 
      +0.95 V for the working potential; 18 kV for the separation voltage; sample 
      injection at 18 kV for 10 s), the compounds investigated can be well separated 
      and detected within 8 min. Excellent linearity was observed between peak currents 
      and concentration of analytes in the range from 0.034 to 70.0 mg/kg for these two 
      compounds. The detection limits (S/N= 3) for enrofloxacin and ciprofloxacin were 
      13.68 mg/kg and 14.35 mg/kg, respectively, which were about 7-fold lower than the 
      maximum residue limits (MRLs) established by the European Union. A simple sample 
      pretreatment method was developed and proved to be effective in obtaining good 
      recoveries and short analysis time. The developed CE-AD method was simpler, 
      faster, and less cost intensive than other reported methods, and allows the 
      determination of ENR and its metabolite CIP in contaminated eel liver samples and 
      other animal tissue samples at the required maximum residue limits.
FAU - Wang, Ling
AU  - Wang L
AD  - Institute of Food Safety and Environmental Monitoring, Fuzhou University, Fuzhou, 
      China.
FAU - Wu, Xiaoping
AU  - Wu X
FAU - Xie, Zenghong
AU  - Xie Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Buffers)
RN  - 0 (Fluoroquinolones)
RN  - 3DX3XEK1BN (Enrofloxacin)
RN  - 5E8K9I0O4U (Ciprofloxacin)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/*analysis
MH  - Antineoplastic Agents/*analysis
MH  - Buffers
MH  - Ciprofloxacin/*analysis
MH  - Eels/metabolism
MH  - Electrophoresis, Capillary
MH  - Enrofloxacin
MH  - Fluoroquinolones/*analysis
MH  - Hydrogen-Ion Concentration
MH  - Liver/metabolism
MH  - Time Factors
EDAT- 2005/08/25 09:00
MHDA- 2006/05/18 09:00
CRDT- 2005/08/25 09:00
PHST- 2005/08/25 09:00 [pubmed]
PHST- 2006/05/18 09:00 [medline]
PHST- 2005/08/25 09:00 [entrez]
AID - 10.1002/jssc.200400110 [doi]
PST - ppublish
SO  - J Sep Sci. 2005 Jul;28(11):1143-8. doi: 10.1002/jssc.200400110.