PMID- 16118510
OWN - NLM
STAT- MEDLINE
DCOM- 20051018
LR  - 20061115
IS  - 0030-3747 (Print)
IS  - 0030-3747 (Linking)
VI  - 37
IP  - 5
DP  - 2005 Sep-Oct
TI  - Effect of alpha-melanocyte-stimulating hormone on interleukin 8 and monocyte 
      chemotactic protein 1 expression in a human retinal pigment epithelial cell line.
PG  - 279-88
AB  - PURPOSE: To examine melanocortin receptor (from MC-1 to MC-5) mRNA and the effect 
      of alpha-melanocyte-stimulating hormone (alpha-MSH) on interleukin 8 (IL-8) and 
      monocyte chemotactic protein 1 (MCP-1) expression in a human retinal pigment 
      epithelial cell line (ARPE-19) stimulated with IL-1beta or tumor necrosis factor 
      alpha (TNF-alpha). METHODS: Expressions of MC-1 to MC-5 mRNA were examined by 
      semiquantitative reverse transcription polymerase chain reaction (RT-PCR). 
      alpha-MSH and IL-1beta or TNF-alpha were added to serum-free medium. IL-8 and 
      MCP-1 mRNA were measured by real-time PCR. IL-8 and MCP-1 protein concentrations 
      were measured using enzyme-linked immunosorbent assay. Nuclear factor kappaB 
      (NF-kappaB) translocation was examined by immunofluorescent staining/microscopy. 
      RESULTS: MC-1 to MC-5 receptor mRNA was expressed in unstimulated cells. IL-1beta 
      stimulated IL-8 and MCP-1 mRNA at 6 h. TNF-alpha stimulated IL-8 and MCP-1 mRNA 
      expression at 1.5 and 3 h. alpha-MSH (10(-14) to 10(-10)M) inhibited IL-8 and 
      MCP-1 mRNA expression in the cells stimulated with IL-1beta or TNF-alpha. 
      alpha-MSH inhibited IL-1beta or TNF-alpha-stimulated IL-8 and MCP-1 protein 
      levels in the media. Immunofluorescent staining/microscopy of NF-kappaB in the 
      nucleus was dense 30 min after stimulation with IL-1beta or TNF-alpha and was 
      decreased by alpha-MSH. CONCLUSIONS: ARPE-19 cells had MC-1 mRNA. alpha-MSH 
      inhibited IL-8 and MCP-1 expression and protein secretion. Possibly, the effect 
      on chemotactic factors may be via suppression of NF-kappaB translocation.
FAU - Cui, Hu-Shan
AU  - Cui HS
AD  - Department of Ophthalmology, Toyama Medical and Pharmaceutical University, 
      Toyama, Japan. ophthal@ms.toyama-mpu.ac.jp
FAU - Hayasaka, Seiji
AU  - Hayasaka S
FAU - Zhang, Xue-Yun
AU  - Zhang XY
FAU - Chi, Zai-Long
AU  - Chi ZL
FAU - Hayasaka, Yoriko
AU  - Hayasaka Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050822
PL  - Switzerland
TA  - Ophthalmic Res
JT  - Ophthalmic research
JID - 0267442
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-8)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Melanocortin)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 581-05-5 (alpha-MSH)
SB  - IM
MH  - Cell Line
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Fluorescent Antibody Technique, Indirect
MH  - *Gene Expression
MH  - Humans
MH  - Interleukin-1/pharmacology
MH  - Interleukin-8/*genetics/metabolism
MH  - NF-kappa B/metabolism
MH  - Pigment Epithelium of Eye/cytology/*drug effects/metabolism
MH  - RNA, Messenger/metabolism
MH  - Receptors, Melanocortin/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/pharmacology
MH  - alpha-MSH/*pharmacology
EDAT- 2005/08/25 09:00
MHDA- 2005/10/19 09:00
CRDT- 2005/08/25 09:00
PHST- 2004/11/17 00:00 [received]
PHST- 2005/02/24 00:00 [accepted]
PHST- 2005/08/25 09:00 [pubmed]
PHST- 2005/10/19 09:00 [medline]
PHST- 2005/08/25 09:00 [entrez]
AID - 87699 [pii]
AID - 10.1159/000087699 [doi]
PST - ppublish
SO  - Ophthalmic Res. 2005 Sep-Oct;37(5):279-88. doi: 10.1159/000087699. Epub 2005 Aug 
      22.