PMID- 1611874
OWN - NLM
STAT- MEDLINE
DCOM- 19920730
LR  - 20190816
IS  - 0305-0491 (Print)
IS  - 0305-0491 (Linking)
VI  - 101
IP  - 4
DP  - 1992 Apr
TI  - Reversed-phase ion-paired HPLC of purine nucleotides from skeletal muscle, heart 
      and brain of the goldfish, Carassius auratus L.--I. Development of the analytical 
      method.
PG  - 577-81
AB  - 1. A reversed-phase ion-paired liquid chromatographic (HPLC) method was developed 
      to measure AMP, ADP, ATP, IMP, NAD+ and NADP+ levels in white muscle, heart and 
      brain of anoxic goldfish. 2. Mobile phase parameters of the HPLC method 
      (concentrations of buffer, organic modifier and counter-ion, and pH) were varied 
      to establish the optimal conditions for separation of the compounds of interest. 
      3. The analytical method was evaluated by calculating some relevant 
      chromatographic parameters (reproducibility and linearity). 4. The HPLC method 
      showed sufficient selectivity, high sensitivity and reproducibility, and 
      excellent linearity.
FAU - Van der Boon, J
AU  - Van der Boon J
AD  - State University of Leiden, Department of Animal Physiology, Gorlaeus 
      Laboratories, The Netherlands.
FAU - de Jong, R L
AU  - de Jong RL
FAU - Van den Thillart, G E
AU  - Van den Thillart GE
FAU - Addink, A D
AU  - Addink AD
LA  - eng
PT  - Journal Article
PL  - England
TA  - Comp Biochem Physiol B
JT  - Comparative biochemistry and physiology. B, Comparative biochemistry
JID - 2984730R
RN  - 0 (Purine Nucleotides)
SB  - IM
MH  - Animals
MH  - *Brain Chemistry
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Goldfish
MH  - Hydrogen-Ion Concentration
MH  - Muscles/*chemistry/metabolism
MH  - Myocardium/*chemistry/metabolism
MH  - Organ Specificity
MH  - Purine Nucleotides/*analysis/metabolism
MH  - Regression Analysis
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
AID - 10.1016/0305-0491(92)90341-n [doi]
PST - ppublish
SO  - Comp Biochem Physiol B. 1992 Apr;101(4):577-81. doi: 
      10.1016/0305-0491(92)90341-n.