PMID- 16120577
OWN - NLM
STAT- MEDLINE
DCOM- 20081103
LR  - 20181201
IS  - 0253-9772 (Print)
IS  - 0253-9772 (Linking)
VI  - 27
IP  - 4
DP  - 2005 Jul
TI  - [Eukaryotic expression and tumor-targeting modification of human mutated-IL-18 
      fusion gene].
PG  - 557-60
AB  - Interleukin-18 (IL-18) is a proinflammatory cytokinin. This protein has a role in 
      regulating immune responses and exhibits significant anti- tumor activities. 
      Epidermal growth factor (EGF) is an important growth factor that plays a central 
      role in the regulation of cell cycle and differentiation. It was proposed that a 
      targeted delivery of IL-18 by generation of IL-18-EGF fusion protein might 
      decrease adverse effects and result in enhancing cytotoxic and antitumor 
      activities. In the present study, a fusion protein, consisting of EGFR binding 
      domain fused to human IL18 mature peptide via a linker peptide of (Gly4ser) 3, 
      was constructed and expressed in the insect cell line Sf9 using Bac-to-Bac 
      baculovirus expression system. We showed that the purified recombinant fusion 
      protein induced similar levels of IFN-gamma to that of native IL-18 protein in 
      human PBMC in the presence of ConA. Furthermore, EGF Receptor competitive test in 
      human epithelial cancer A431 cell line showed that EGF- IL18 fusion protein can 
      specifically bind with EGFR by competing with native EGF protein. The results 
      suggested that EGF-IL18 fusion protein could specifically be targeted on tumor 
      cells. The work may provide a new insight into the cytokinin for the tumor 
      treatment.
FAU - Lu, Jian-Xin
AU  - Lu JX
AD  - Institute of Cellular and Molecular Medicine, Wenzhou Medical College, Wenzhou 
      325035, China. jx@wz.zj.cn
FAU - Peng, Ying
AU  - Peng Y
FAU - Meng, Zhe-Feng
AU  - Meng ZF
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Yi Chuan
JT  - Yi chuan = Hereditas
JID - 9436478
RN  - 0 (Interleukin-18)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 82115-62-6 (Interferon-gamma)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Animals
MH  - Baculoviridae/genetics
MH  - Binding, Competitive
MH  - Blotting, Western
MH  - Cell Line
MH  - Cell Line, Tumor
MH  - Drug Delivery Systems
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epidermal Growth Factor/genetics/*metabolism
MH  - ErbB Receptors/*metabolism
MH  - Humans
MH  - Interferon-gamma/metabolism
MH  - Interleukin-18/genetics/*metabolism
MH  - Recombinant Fusion Proteins/administration & dosage/genetics/*metabolism
MH  - Spodoptera
EDAT- 2005/08/27 09:00
MHDA- 2008/11/04 09:00
CRDT- 2005/08/27 09:00
PHST- 2005/08/27 09:00 [pubmed]
PHST- 2008/11/04 09:00 [medline]
PHST- 2005/08/27 09:00 [entrez]
AID - 0253-9772(2005)04-0557-04 [pii]
PST - ppublish
SO  - Yi Chuan. 2005 Jul;27(4):557-60.