PMID- 16120747
OWN - NLM
STAT- MEDLINE
DCOM- 20051115
LR  - 20161124
IS  - 1096-6080 (Print)
IS  - 1096-0929 (Linking)
VI  - 88
IP  - 1
DP  - 2005 Nov
TI  - Toxicogenomic profile of 2,3,7,8-tetrachlorodibenzo-p-dioxin in the murine fetal 
      heart: modulation of cell cycle and extracellular matrix genes.
PG  - 231-41
AB  - 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) and similar environmental contaminants 
      have been demonstrated to be potent cardiovascular teratogens in developing 
      piscine and avian species. In the present study, we investigated the effects of 
      TCDD on gene expression during murine cardiovascular development. C57Bl6N 
      pregnant mice were dosed with 1.5, 3.0, or 6.0 microg TCDD/kg on gestational day 
      (GD) 14.5, and microarray analysis was used to characterize the global changes in 
      fetal cardiac gene expression on GD 17.5. TCDD significantly altered expression 
      of a number of genes involved in xenobiotic metabolism, cardiac homeostasis, 
      extracellular matrix production/remodeling, and cell cycle regulation. 
      Interestingly, while the AhR-responsive genes Cyp1A1, Cyp1B1, Ugt1a6, and Ahrr, 
      were all induced by TCDD in the fetal murine heart, other AhR-responsive genes, 
      Cyp1a2, Nqo1, and Gsta1, were not. Quantitative real-time polymerase chain 
      reactions confirmed the changes in expression of several G1/S-type cyclins and 
      extracellular matrix-related genes. These results demonstrate the global changes 
      in cardiac gene expression that result from TCDD exposure of the fetal murine 
      heart and implicate genes involved in cell cycle and extracellular matrix 
      regulation in TCDD-induced cardiac teratogenicity and functional deficits.
FAU - Thackaberry, E A
AU  - Thackaberry EA
AD  - College of Pharmacy, University of New Mexico Health Sciences Center, 
      Albuquerque, New Mexico 87131, USA.
FAU - Jiang, Z
AU  - Jiang Z
FAU - Johnson, C D
AU  - Johnson CD
FAU - Ramos, K S
AU  - Ramos KS
FAU - Walker, M K
AU  - Walker MK
LA  - eng
GR  - ES012335/ES/NIEHS NIH HHS/United States
GR  - ES012855/ES/NIEHS NIH HHS/United States
GR  - P30 ES12072/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050824
PL  - United States
TA  - Toxicol Sci
JT  - Toxicological sciences : an official journal of the Society of Toxicology
JID - 9805461
RN  - 0 (Environmental Pollutants)
RN  - 0 (Polychlorinated Dibenzodioxins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Aryl Hydrocarbon)
RN  - 0 (Teratogens)
SB  - IM
MH  - Animals
MH  - Cell Cycle/*drug effects/genetics
MH  - Dose-Response Relationship, Drug
MH  - Environmental Pollutants/*toxicity
MH  - Extracellular Matrix/*drug effects/genetics
MH  - Female
MH  - Fetal Development
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Developmental/*drug effects
MH  - Heart/*drug effects/embryology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Myocardium/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Polychlorinated Dibenzodioxins/*toxicity
MH  - Pregnancy
MH  - RNA, Messenger/metabolism
MH  - Receptors, Aryl Hydrocarbon/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Teratogens/*toxicity
EDAT- 2005/08/27 09:00
MHDA- 2005/11/16 09:00
CRDT- 2005/08/27 09:00
PHST- 2005/08/27 09:00 [pubmed]
PHST- 2005/11/16 09:00 [medline]
PHST- 2005/08/27 09:00 [entrez]
AID - kfi301 [pii]
AID - 10.1093/toxsci/kfi301 [doi]
PST - ppublish
SO  - Toxicol Sci. 2005 Nov;88(1):231-41. doi: 10.1093/toxsci/kfi301. Epub 2005 Aug 24.