PMID- 16121249
OWN - NLM
STAT- MEDLINE
DCOM- 20070424
LR  - 20161020
IS  - 0831-2796 (Print)
IS  - 0831-2796 (Linking)
VI  - 48
IP  - 3
DP  - 2005 Jun
TI  - A high-resolution karyotype of cucumber (Cucumis sativus L. 'Winter Long') 
      revealed by C-banding, pachytene analysis, and RAPD-aided fluorescence in situ 
      hybridization.
PG  - 534-40
AB  - Using molecular cytogenetic DNA markers, C-banding, pachytene analysis, and 
      fluorescence in situ hybridization (FISH), a high-resolution karyotype was 
      established in the cucumber. C-banding showed distinct hetero chromatic bands on 
      the pericentromeric, telomeric, and intercalary regions of the chromosomes. The 
      C-banding patterns were also consistent with the morphology of 
      4'-6-diamino-2-phenylindole dihydrochloride (DAPI)-stained pachytene chromosomes. 
      Two repetitive DNA fragments, CsRP1 and CsRP2, were obtained by PCR and localized 
      on the mitotic metaphase and meiotic pachytene chromosomes. CsRP1 was detected on 
      the pericentromeric heterochromatic regions of all chromosomes, except chromosome 
      1. CsRP2 was detected on 5 (chromosomes 1, 2, 3, 4, and 7) of 7 chromosomes. All 
      homologous chromosome pairs could be distinguished by FISH using 2 RAPD markers. 
      This is the first report on molecular karyotyping of mitotic and meiotic spreads 
      of cucumber.
FAU - Koo, Dal-Hoe
AU  - Koo DH
AD  - School of Bioscience and Biotechnology, Chungnam National University, Korea.
FAU - Choi, Hae-Woon
AU  - Choi HW
FAU - Cho, Jeongki
AU  - Cho J
FAU - Hur, Yoonkang
AU  - Hur Y
FAU - Bang, Jae-Wook
AU  - Bang JW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Genetic Markers)
RN  - 0 (Indoles)
RN  - 0 (RNA, Ribosomal, 5S)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Base Sequence
MH  - Cucumis sativus/*genetics
MH  - DNA, Ribosomal/genetics
MH  - Genetic Markers
MH  - In Situ Hybridization, Fluorescence
MH  - Indoles
MH  - Karyotyping
MH  - Metaphase/genetics
MH  - Molecular Sequence Data
MH  - Pachytene Stage/*genetics
MH  - RNA, Ribosomal, 5S/genetics
MH  - Random Amplified Polymorphic DNA Technique
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 2005/08/27 09:00
MHDA- 2007/04/25 09:00
CRDT- 2005/08/27 09:00
PHST- 2005/08/27 09:00 [pubmed]
PHST- 2007/04/25 09:00 [medline]
PHST- 2005/08/27 09:00 [entrez]
AID - g04-128 [pii]
AID - 10.1139/g04-128 [doi]
PST - ppublish
SO  - Genome. 2005 Jun;48(3):534-40. doi: 10.1139/g04-128.