PMID- 16121337
OWN - NLM
STAT- MEDLINE
DCOM- 20060123
LR  - 20061115
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 5
IP  - 14
DP  - 2005 Sep
TI  - Sub-proteome analysis of novel IgE-binding proteins from Bermuda grass pollen.
PG  - 3805-13
AB  - Bermuda grass (Cynodon dactylon) pollen (BGP) is one of the most common causes of 
      airway allergic disease, and has been shown to contain over 12 allergenic 
      proteins on 1-D immunoglobulin E (IgE) immunoblots. However, only a few allergens 
      have been identified and characterized. Cyn d 1 is a major allergen and the most 
      abundant protein in BGP, representing 15% of the whole-pollen extract. To 
      investigate variability in the IgE-reactive patterns of BGP-sensitized patients 
      and to identify other prevalent allergens, a BGP extract was passed through an 
      affinity column to remove Cyn d 1, and the non-bound material was collected and 
      analyzed by 2-DE. IgE-reactive proteins were subsequently characterized by 
      immunoblotting using serum samples from ten BGP-allergic patients. The prevalent 
      IgE-reactive proteins were identified by MALDI-TOF MS, N-terminal sequence 
      similarity, and LC-MS/MS. Here, we present a sub-proteome approach for allergen 
      investigation and its use for determining BGP 2-DE profiles and identifying six 
      novel allergens.
FAU - Kao, Shao-Hsuan
AU  - Kao SH
AD  - Institute of Biochemistry and Molecular Biology, College of Medicine, National 
      Taiwan University, Taipei, Taiwan.
FAU - Su, Song-Nan
AU  - Su SN
FAU - Huang, Shih-Wen
AU  - Huang SW
FAU - Tsai, Jaw-Ji
AU  - Tsai JJ
FAU - Chow, Lu-Ping
AU  - Chow LP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Plant Proteins)
RN  - 0 (Proteome)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Blotting, Western
MH  - Cynodon/*chemistry
MH  - Immunoglobulin E/*metabolism
MH  - Plant Proteins/*chemistry/metabolism
MH  - *Proteome
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
EDAT- 2005/08/27 09:00
MHDA- 2006/01/24 09:00
CRDT- 2005/08/27 09:00
PHST- 2005/08/27 09:00 [pubmed]
PHST- 2006/01/24 09:00 [medline]
PHST- 2005/08/27 09:00 [entrez]
AID - 10.1002/pmic.200401229 [doi]
PST - ppublish
SO  - Proteomics. 2005 Sep;5(14):3805-13. doi: 10.1002/pmic.200401229.