PMID- 16123570
OWN - NLM
STAT- MEDLINE
DCOM- 20051208
LR  - 20141120
IS  - 0042-1138 (Print)
IS  - 0042-1138 (Linking)
VI  - 75
IP  - 2
DP  - 2005
TI  - Intermittent pressure-loading increases transforming growth factor-beta-1 
      secretion from renal tubular epithelial cells: in vitro vesicoureteral reflux 
      model.
PG  - 150-8
AB  - BACKGROUND: To investigate the effect of hydrodynamic pressure mimicking 
      vesicoureteral reflux on renal tubular epithelial cells in vitro, we constructed 
      an intermittent pressure-loading (IPL) model of Madin-Darby canine kidney (MDCK) 
      cells. MATERIALS AND METHODS: Three grades of pressure were loaded onto the MDCK 
      cells intermittently. The concentration of cytokines in the supernatant, the 
      amount of the protein and its mRNA in the MDCK cells were studied, respectively. 
      RESULTS: After 24 h, the concentration of transforming growth factor-beta1 
      (TGF-beta1) increased under intense IPL conditions (100 and 200 cm H2O) in the 
      15-min IPL group (p<0.05, p<0.01). The amount of cellular level of TGF-beta1 
      protein and its mRNA did not show any significant increase within 24 h under the 
      present conditions. The concentration of monocyte chemoattractant peptide-1 
      (MCP-1) was not significantly different from that of the control. CONCLUSION: 
      These data suggest that the early TGF-beta1 secretion phenomenon without change 
      in gene expression is the case in the renal tubular epithelial cells under 
      certain intermittent pressure-loading conditions.
CI  - Copyright (c) 2005 S. Karger AG, Basel.
FAU - Maruyama, Tetsuji
AU  - Maruyama T
AD  - Department of Nephro-Urology, Nagoya City University Graduate School of Medical 
      Sciences, Nagoya, Japan. tmaru@med.nagoya-cu.ac.jp
FAU - Hayashi, Yutaro
AU  - Hayashi Y
FAU - Nakane, Akihiro
AU  - Nakane A
FAU - Sasaki, Shoichi
AU  - Sasaki S
FAU - Kohri, Kenjiro
AU  - Kohri K
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Urol Int
JT  - Urologia internationalis
JID - 0417373
RN  - 0 (Chemokine CCL2)
RN  - 0 (Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Blotting, Western
MH  - Cell Survival
MH  - Cells, Cultured
MH  - Chemokine CCL2/analysis/*biosynthesis
MH  - Dogs
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/cytology/*physiology
MH  - In Vitro Techniques
MH  - Kidney Tubules/*cytology
MH  - Models, Animal
MH  - *Pressure
MH  - RNA/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Stress, Mechanical
MH  - Transforming Growth Factor beta/analysis/*biosynthesis
MH  - Transforming Growth Factor beta1
MH  - Vesico-Ureteral Reflux
EDAT- 2005/08/27 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/27 09:00
PHST- 2004/06/01 00:00 [received]
PHST- 2005/03/01 00:00 [accepted]
PHST- 2005/08/27 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/27 09:00 [entrez]
AID - 87170 [pii]
AID - 10.1159/000087170 [doi]
PST - ppublish
SO  - Urol Int. 2005;75(2):150-8. doi: 10.1159/000087170.