PMID- 16124465
OWN - NLM
STAT- MEDLINE
DCOM- 20061219
LR  - 20161018
IS  - 0250-3301 (Print)
IS  - 0250-3301 (Linking)
VI  - 26
IP  - 3
DP  - 2005 May
TI  - [Free radical generation and lipid peroxidation induced by 2,4-dichlorophenol in 
      liver of Carassius auratus].
PG  - 29-32
AB  - Free radical generation in liver of larval fish (Carassius auratus) after 
      2,4-dichlorophenol (2,4-DCP) injected intraperitoneally 24 h was studied with 
      electron paramagnetic resonance (EPR) spin trapping technique and 
      alpha-phenyl-N-tert-butylnitrone (PBN) as spin trap reagent. The results show 
      that there is a significant increase of free radical generation in the liver of 
      the fish treated with 2,4-DCP (0.025, 0.05, 0.5, 5, 25 mg/kg) compared with that 
      of the control (p<0.05). The free radical generation correlates well with the 
      dose of 2,4-DCP administered. The regress equation for dose-effect relation is y 
      = 1189ln(x) + 9220, r2 = 0.9856. The six-line composed of three groups with two 
      hyperfine splitting peaks in each shows a typical PBN/*OH EPR spectrum. The 
      hyperfine splitting constants for the PBN-adducts is alphaN = 13.7 Gauss, alphaH= 
      1.8 Gauss, g factor is 2.0058, which is consistent with the characteristics of 
      PBN-hydroxyl radical adduct reported from the literature. Results also show that 
      there is a significant increase for malondialdehyde (MDA) content in the treated 
      groups compared with that of the control (p<0.05). The mechanisms of free radical 
      generation and lipid peroxidation induced by 2,4-DCP are discussed in this study.
FAU - Luo, Yi
AU  - Luo Y
AD  - State Key Laboratory of Pollution Control and Resources Reuse, School of 
      Environment, Nanjing University, Nanjing 210093, China. yiluo@lnu.edu.cn
FAU - Shi, Hua-hong
AU  - Shi HH
FAU - Wang, Xiao-rong
AU  - Wang XR
FAU - Ji, Liang-liang
AU  - Ji LL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Huan Jing Ke Xue
JT  - Huan jing ke xue= Huanjing kexue
JID - 8405344
RN  - 0 (Chlorophenols)
RN  - 0 (Free Radicals)
RN  - R669TG1950 (2,4-dichlorophenol)
SB  - IM
MH  - Animals
MH  - Chlorophenols/*toxicity
MH  - Free Radicals/*metabolism
MH  - Goldfish/*metabolism
MH  - Lipid Peroxidation/drug effects
MH  - Liver/*metabolism
EDAT- 2005/08/30 09:00
MHDA- 2006/12/21 09:00
CRDT- 2005/08/30 09:00
PHST- 2005/08/30 09:00 [pubmed]
PHST- 2006/12/21 09:00 [medline]
PHST- 2005/08/30 09:00 [entrez]
PST - ppublish
SO  - Huan Jing Ke Xue. 2005 May;26(3):29-32.