PMID- 16124574
OWN - NLM
STAT- MEDLINE
DCOM- 20070619
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 23
IP  - 3
DP  - 2005 May
TI  - [Determination of ligustrazine in human plasma by high performance liquid 
      chromatography with solid-phase extraction].
PG  - 276-8
AB  - A high performance liquid chromatographic method was developed for the 
      determination of ligustrazine in human plasma. The chromatographic separation was 
      performed on a Luna C18 column (150 mm x 4.6 mm i.d., 5 microm) at column 
      temperature of 40 degrees C. The mobile phase, a mixture of 
      methanol-acetonitrile-acetate buffer of pH 5.0 (50:8:42, v/v), was delivered at a 
      flow rate of 1.0 mL/min. The detection wavelength was 280 nm. Plasma samples were 
      prepared with a C8 solid-phase extraction column. Linearity was confirmed in the 
      mass concentration range of 25-5000 microg/L with the correlation coefficient of 
      0.9999. The extraction recovery of ligustrazine ranged from 96.72% to 100.90%. 
      The relative standard deviations (RSDs) of intra- and inter-day assay at the mass 
      concentrations of 50, 500 and 3000 microg/L were less than 8.64% and the 
      accuracies were between 99.59%-103.26%. The limit of detection (LOD) was 10 
      microg/L. The results of this method validation satisfactorily meet the 
      acceptance criteria of bioanalysis and the method is applicable to the 
      pharmacokinetic studies of ligustrazine in human beings.
FAU - Weng, Weiyu
AU  - Weng W
AD  - School of Pharmacy, Fudan University, Shanghai 200032, China. wyweng@shmu.edu.cn
FAU - Shen, Teng
AU  - Shen T
FAU - Xu, Huinan
AU  - Xu H
FAU - Zhang, Jianfang
AU  - Zhang J
FAU - Huang, Jianming
AU  - Huang J
FAU - Huang, Zhaochang
AU  - Huang Z
FAU - Cai, Jia
AU  - Cai J
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Pyrazines)
RN  - V80F4IA5XG (tetramethylpyrazine)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Humans
MH  - Pyrazines/*blood
MH  - Solid Phase Extraction/*methods
EDAT- 2005/08/30 09:00
MHDA- 2007/06/20 09:00
CRDT- 2005/08/30 09:00
PHST- 2005/08/30 09:00 [pubmed]
PHST- 2007/06/20 09:00 [medline]
PHST- 2005/08/30 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2005 May;23(3):276-8.