PMID- 16125217
OWN - NLM
STAT- MEDLINE
DCOM- 20060131
LR  - 20211203
IS  - 0090-8258 (Print)
IS  - 0090-8258 (Linking)
VI  - 99
IP  - 3
DP  - 2005 Dec
TI  - Array comparative genomic hybridization analysis of uterine leiomyosarcoma.
PG  - 545-51
AB  - PURPOSE: Using a genome-wide array-based comparative genomic hybridization 
      (array-CGH), DNA copy number changes in uterine leiomyosarcoma were analyzed. 
      MATERIALS AND METHODS: We analyzed 4 cases of uterine leiomyoma and 7 cases of 
      uterine leiomyosarcoma. The paraffin-fixed tissue samples were microdissected 
      under microscope and DNA was extracted. Array-based CGH and fluorescence in situ 
      hybridization (FISH) were carried out with Genome database (Gene Ontology). 
      RESULTS: Uterine leiomyoma showed no genetic alterations, while all of 7 cases of 
      uterine leiomyosarcoma showed specific gains and losses. The percentage of 
      average gains and losses were 4.86% and 15.1%, respectively. The regions of high 
      level of gain were 7q36.3, 7q33-q35, 12q13-12q15, and 12q23.3. And the regions of 
      homozygous loss were 1p21.1, 2p22.2, 6p11.2, 9p21.1, 9p21.3, 9p22.1, 14q32.33, 
      and 14q32.33 qter. There were no recurrent regions of gain, but recurrent regions 
      of loss were 1p21.1-p21.2, 1p22.3-p31.1, 9p21.2-p22.2, 10q25-q25.2, 11q24.2-q25, 
      13q12-q12.13, 14q31.1-q31.3, 14q32.32-q32.33, 15q11-q12, 15q13-q14, 
      18q12.1-q12.2, 18q22.1-q22.3, 20p12.1, and 21q22.12-q22.13. In the high level of 
      gain regions, BAC clones encoded HMGIC, SAS, MDM2, TIM1 genes. Frequently gained 
      BAC clone-encoded genes were TIM1, PDGFR-beta, REC Q4, VAV2, FGF4, KLK2, PNUTL1, 
      GDNF, FLG, EXT1, WISP1, HER-2, and SOX18. The genes encoded by frequently lost 
      BAC clones were LEU1, ERCC5, THBS1, DCC, MBD2, SCCA1, FVT1, CYB5, and ETS2/E2. A 
      subset of cellular processes from each gene was clustered by Gene Ontology 
      database. CONCLUSION: Using array-CGH, chromosomal aberrations related to uterine 
      leiomyosarcoma were identified. The high resolution of array-CGH combined with 
      human genome database would give a chance to find out possible target genes 
      present in the gained or lost clones.
FAU - Cho, Young Lae
AU  - Cho YL
AD  - Department of Obstetrics and Gynecology, Kyungpook National University, Daegu, 
      Republic of Korea.
FAU - Bae, SuMi
AU  - Bae S
FAU - Koo, Myeong Suk
AU  - Koo MS
FAU - Kim, Kyung Mee
AU  - Kim KM
FAU - Chun, Heung-Jae
AU  - Chun HJ
FAU - Kim, Chong Kook
AU  - Kim CK
FAU - Ro, Duck Young
AU  - Ro DY
FAU - Kim, Jang Heub
AU  - Kim JH
FAU - Lee, Chang-Hun
AU  - Lee CH
FAU - Kim, Yong-Wan
AU  - Kim YW
FAU - Ahn, Woong Shick
AU  - Ahn WS
LA  - eng
PT  - Journal Article
DEP - 20050824
PL  - United States
TA  - Gynecol Oncol
JT  - Gynecologic oncology
JID - 0365304
RN  - 0 (DNA, Neoplasm)
RN  - 0 (FLG protein, human)
RN  - 0 (Filaggrin Proteins)
SB  - IM
MH  - Adult
MH  - *Chromosome Aberrations
MH  - DNA, Neoplasm/genetics
MH  - Female
MH  - Filaggrin Proteins
MH  - Gene Deletion
MH  - Gene Dosage
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leiomyosarcoma/*genetics
MH  - Middle Aged
MH  - Nucleic Acid Hybridization/methods
MH  - Uterine Neoplasms/*genetics
EDAT- 2005/08/30 09:00
MHDA- 2006/02/01 09:00
CRDT- 2005/08/30 09:00
PHST- 2005/01/12 00:00 [received]
PHST- 2005/07/16 00:00 [revised]
PHST- 2005/07/18 00:00 [accepted]
PHST- 2005/08/30 09:00 [pubmed]
PHST- 2006/02/01 09:00 [medline]
PHST- 2005/08/30 09:00 [entrez]
AID - S0090-8258(05)00601-3 [pii]
AID - 10.1016/j.ygyno.2005.07.017 [doi]
PST - ppublish
SO  - Gynecol Oncol. 2005 Dec;99(3):545-51. doi: 10.1016/j.ygyno.2005.07.017. Epub 2005 
      Aug 24.