PMID- 16125224
OWN - NLM
STAT- MEDLINE
DCOM- 20060321
LR  - 20181201
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 27
IP  - 6
DP  - 2006 Feb
TI  - Bone tissue engineering evaluation based on rat calvaria stromal cells cultured 
      on modified PLGA scaffolds.
PG  - 896-904
AB  - Using natural materials to coat the scaffolds used for tissue-engineered 
      bone-repair techniques is expected to increase osteoblast adhesion to the 
      scaffold and to express normal physiological function. To test this hypothesis, 
      we therefore modified poly(DL-lactic-co-glycolic acid) (PLGA) substrate by 
      coating it with natural biomaterial solutions of collagen, chitosan, or 
      N-succinyl-chitosan, and then used these three combinations as scaffolds to 
      evaluate their effects on osteoblast attachment, proliferation, and 
      differentiation. The results demonstrated that the pore size of scaffolds ranging 
      from 125-500 microm did not affect the osteoblast phenotype; however, the surface 
      modification of the scaffolds coated with these natural biomaterials did. 
      Collagen increased cell attachment and proliferation, but chitosan and 
      N-succinyl-chitosan decreased them. Chitosan and N-succinyl-chitosan increased 
      differentiation, but collagen decreased it. These results provide us a new 
      strategy for modifying microenvironments to increase osteoblast adhesion, 
      proliferation, and differentiation on PLGA scaffolds, a strategy that might be 
      useful for tissue regeneration.
FAU - Wu, Yu-Chun
AU  - Wu YC
AD  - Institute of Biotechnology, National Cheng Kung University, Tainan 70428, Taiwan.
FAU - Shaw, Shyh-Yu
AU  - Shaw SY
FAU - Lin, Hong-Ru
AU  - Lin HR
FAU - Lee, Tzer-Min
AU  - Lee TM
FAU - Yang, Chyun-Yu
AU  - Yang CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050825
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Polymers)
RN  - 059QF0KO0R (Water)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Animals
MH  - Cell Adhesion
MH  - Cell Culture Techniques/*methods
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Lactic Acid/*chemistry
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/cytology
MH  - Polyglycolic Acid/*chemistry
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Polymers/*chemistry
MH  - Rats
MH  - Skull/*cytology
MH  - Stromal Cells/*cytology
MH  - *Tissue Engineering
MH  - Water/chemistry
EDAT- 2005/08/30 09:00
MHDA- 2006/03/22 09:00
CRDT- 2005/08/30 09:00
PHST- 2005/03/25 00:00 [received]
PHST- 2005/07/01 00:00 [accepted]
PHST- 2005/08/30 09:00 [pubmed]
PHST- 2006/03/22 09:00 [medline]
PHST- 2005/08/30 09:00 [entrez]
AID - S0142-9612(05)00603-4 [pii]
AID - 10.1016/j.biomaterials.2005.07.002 [doi]
PST - ppublish
SO  - Biomaterials. 2006 Feb;27(6):896-904. doi: 10.1016/j.biomaterials.2005.07.002. 
      Epub 2005 Aug 25.