PMID- 16125559
OWN - NLM
STAT- MEDLINE
DCOM- 20051202
LR  - 20220318
IS  - 0093-691X (Print)
IS  - 0093-691X (Linking)
VI  - 64
IP  - 5
DP  - 2005 Sep 15
TI  - Increase of ICSI efficiency with hyaluronic acid binding sperm for low aneuploidy 
      frequency in pig.
PG  - 1158-69
AB  - The present study was designed to evaluate the ability of hyaluronic acid binding 
      sperm (HABS) in increasing the efficiency of intracytoplasmic sperm injection 
      (ICSI) in terms of the production of chromosomally normal porcine embryos. 
      Porcine embryos were produced by in vitro fertilization (IVF), ICSI and ICSI 
      using hyaluronic acid binding sperm (ICSI-HABS). Chromosome aneuploidy in sperm 
      and embryos was evaluated using chromosome 1 submetacentric probe for 
      fluorescence in situ hybridization (FISH) analysis. No significant differences 
      were observed in the blastocysts rates (18.6, 23.6 and 23.8%) and cell numbers 
      (61.8+/-12.5, 55.5+/-7.3 and 59.3+/-9.6) among embryos derived from IVF, ICSI, 
      and ICSI-HABS. However, the frequency of normal diploidy in ICSI-HABS (75.5%) was 
      significantly higher (P<0.05) than that in IVF (57.0%) and ICSI (68.2%). Embryos 
      from ICSI-HABS showed significantly lower chromosome abnormality rate (P<0.05). 
      Both ICSI and IVF embryos showed higher rates of polyploidy, and hence 
      chromosomally abnormal embryos, in comparison to ICSI-HABS embryos. In addition, 
      we investigated the chromosomal complement of porcine spermatozoa by FISH. The 
      rate of chromosome number abnormality in porcine sperm was found to be 6.25% 
      (70/1120). Thus, we conclude that the use of hyaluronic acid binding sperm is 
      superior to morphological sperm selection for ICSI in producing chromosomally 
      normal embryos and increasing the ICSI efficiency by lowering the aneuploidy 
      frequency. Our results indicate that the selection of normal sperm with 
      hyaluronic acid binding assay might help to reduce the early embryonic mortality 
      due to chromosomal aneuploidy thereby increasing the success rate of embryo 
      transfer technology in pigs.
FAU - Park, Chun Young
AU  - Park CY
AD  - Department of Animal Science and Biotechnology, Konkuk University, 1 
      Hwayang-dong, Gwangjin-gu, Seoul 143-701, Republic of Korea.
FAU - Uhm, Sang Jun
AU  - Uhm SJ
FAU - Song, Sang Jin
AU  - Song SJ
FAU - Kim, Kwang Sung
AU  - Kim KS
FAU - Hong, Seung Bum
AU  - Hong SB
FAU - Chung, Kil Saeng
AU  - Chung KS
FAU - Park, Chankyu
AU  - Park C
FAU - Lee, Hoon Taek
AU  - Lee HT
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050402
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - *Aneuploidy
MH  - Animals
MH  - Chromosome Aberrations/embryology/veterinary
MH  - Embryo Transfer/veterinary
MH  - Female
MH  - Fertilization in Vitro/veterinary
MH  - Hyaluronic Acid/*metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Pregnancy
MH  - Sperm Injections, Intracytoplasmic/*veterinary
MH  - Spermatozoa/*metabolism/ultrastructure
MH  - Swine/embryology/genetics
MH  - Swine Diseases/*genetics
EDAT- 2005/08/30 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/08/30 09:00
PHST- 2004/10/25 00:00 [received]
PHST- 2004/12/23 00:00 [revised]
PHST- 2005/01/31 00:00 [accepted]
PHST- 2005/08/30 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/08/30 09:00 [entrez]
AID - S0093-691X(05)00039-7 [pii]
AID - 10.1016/j.theriogenology.2005.01.010 [doi]
PST - ppublish
SO  - Theriogenology. 2005 Sep 15;64(5):1158-69. doi: 
      10.1016/j.theriogenology.2005.01.010. Epub 2005 Apr 2.