PMID- 16126998
OWN - NLM
STAT- MEDLINE
DCOM- 20060626
LR  - 20191210
IS  - 0077-8923 (Print)
IS  - 0077-8923 (Linking)
VI  - 1051
DP  - 2005 Jun
TI  - Comparative analysis of antigen specificities in the monomeric and dimeric 
      fractions of intravenous immunoglobulin.
PG  - 582-90
AB  - Intravenous immunoglobulin (IVIG) preparations are derived from the pooled plasma 
      of thousands of healthy donors and contain a complex mix of antibodies. Depending 
      on the formulation, IVIG preparations contain variable amounts of monomeric and 
      dimeric IgG. The biological and therapeutic significance of these IVIG fractions 
      is still ill defined. Kinetic analysis of monomeric and dimeric IgG isolated by 
      size-exclusion chromatography revealed a stable monomeric versus an unstable 
      dimeric IgG fraction tending to dissociation. Biochemical analysis by 2D gel 
      electrophoresis and isotype analysis showed no significant differences between 
      the fractions. In contrast, comparative analysis by immunodot, ELISA, FACS, and 
      immunohistology of monomeric and dimeric IgG fractions showed a preferential 
      reactivity of the dimeric IgG on a variety of both self-antigens and exoantigens.
FAU - Miescher, Sylvia M
AU  - Miescher SM
AD  - ZLB Behring AG, Wankdorfstrasse 10, 3000 Bern 22, Switzerland. 
      sylvia.miescher@zlb.com
FAU - Schaub, Alexander
AU  - Schaub A
FAU - Ghielmetti, Marco
AU  - Ghielmetti M
FAU - Baumann, Michael
AU  - Baumann M
FAU - Vogel, Monique
AU  - Vogel M
FAU - Bolli, Reinhard
AU  - Bolli R
FAU - Stadler, Beda
AU  - Stadler B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann N Y Acad Sci
JT  - Annals of the New York Academy of Sciences
JID - 7506858
RN  - 0 (Epitopes)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Immunoglobulins, Intravenous)
SB  - IM
MH  - Animals
MH  - CHO Cells
MH  - COS Cells
MH  - Chlorocebus aethiops
MH  - Cricetinae
MH  - Dimerization
MH  - Epitopes
MH  - Humans
MH  - Immunoglobulin G/chemistry/immunology
MH  - Immunoglobulins, Intravenous/chemistry/*immunology
EDAT- 2005/08/30 09:00
MHDA- 2006/06/27 09:00
CRDT- 2005/08/30 09:00
PHST- 2005/08/30 09:00 [pubmed]
PHST- 2006/06/27 09:00 [medline]
PHST- 2005/08/30 09:00 [entrez]
AID - 1051/1/582 [pii]
AID - 10.1196/annals.1361.102 [doi]
PST - ppublish
SO  - Ann N Y Acad Sci. 2005 Jun;1051:582-90. doi: 10.1196/annals.1361.102.