PMID- 16130755
OWN - NLM
STAT- MEDLINE
DCOM- 20051028
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1088
IP  - 1-2
DP  - 2005 Sep 23
TI  - Routine application using single quadrupole liquid chromatography-mass 
      spectrometry to pesticides analysis in citrus fruits.
PG  - 224-33
AB  - A rapid and sensitive liquid chromatography-electrospray ionization-mass 
      spectrometry method has been developed for the routine analysis of buprofezin, 
      bupirimate, hexaflumuron, tebufenpyrad, fluvalinate and pyriproxyfen in citrus 
      fruits. Extracts were obtained by matrix solid-phase dispersion (MSPD) using C18 
      as dispersant and dichloromethane-methanol (80:20, v/v) as eluent. Matrix effects 
      were tested for all matrices by addition of standard to sample blank extracts 
      (samples containing no detectable residues). Mean recoveries obtained at 
      fortification levels between 0.01 and 5 mg kg(-1) were 57-97% with relative 
      standard deviations (RSDs) from 5 to 19%. The limits of quantification (LOQ) were 
      in the range of 0.01-0.2 mg kg(-1) and lower than maximum residue limits (MRLs) 
      established by the Spanish legislation. The MSPD was compared with conventional 
      ethyl acetate extraction, showing equivalent recoveries and precision. Although 
      the sample is more concentrated (5-fold) by solid-liquid extraction (SLE) with 
      ethyl acetate than by MSPD, LOQs obtained by both techniques, were almost equal, 
      because MSPD reduces matrix effects, baseline noise, and interfering peaks from 
      the matrix. The proposed method has been applied to the determination of selected 
      pesticides in real samples. Liquid chromatography-tandem mass spectrometry 
      (LC-MS-MS) with quadrupole ion trap (QIT) and triple quadrupole (TQ) have been 
      used as confirmatory tool for positive samples according to a recent No. 
      SANCO/10476/2003 European Union Guideline.
FAU - Soler, Carla
AU  - Soler C
AD  - Laboratori de Bromatologia i Toxicologia, Facultat de Farmacia, Universitat de 
      Valencia, Burjassot, Valencia, Spain. carla.soler@uv.es
FAU - Manes, Jordi
AU  - Manes J
FAU - Pico, Yolanda
AU  - Pico Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Pesticides)
SB  - IM
MH  - Chromatography, Liquid/*methods
MH  - Fruit/*chemistry
MH  - Pesticides/*analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2005/09/01 09:00
MHDA- 2005/10/29 09:00
CRDT- 2005/09/01 09:00
PHST- 2005/09/01 09:00 [pubmed]
PHST- 2005/10/29 09:00 [medline]
PHST- 2005/09/01 09:00 [entrez]
AID - S0021-9673(05)00658-8 [pii]
AID - 10.1016/j.chroma.2005.03.106 [doi]
PST - ppublish
SO  - J Chromatogr A. 2005 Sep 23;1088(1-2):224-33. doi: 10.1016/j.chroma.2005.03.106.