PMID- 16130901
OWN - NLM
STAT- MEDLINE
DCOM- 20051107
LR  - 20191109
IS  - 0914-7470 (Print)
IS  - 0914-7470 (Linking)
VI  - 18
IP  - 1
DP  - 2005 Mar
TI  - Establishment and characterization of human glioblastoma cell line (HUBT-n).
PG  - 59-65
AB  - We succeeded in primary culture of 3 in 4 cases of glioblastomas. The long-term 
      passage cultures were not done from the primary cultures of original tumor, but 
      glioblastoma cell line (HUBT-n) was established from a xenograft of nude mouse. 
      This line grew well without interruption for 4 years and was subcultivated over 
      120 times. The cells were spindle like or round in shape and neoplastic and 
      pleomorphic features contained glial fibrillar acid protein (GFAP) and S-100 
      protein and grew multilayering without contact inhibition. A bough-shaped long 
      projection was noted from a small cell. One of the characteristics of the HUBT-n 
      cells was existence of well developed intermediate filaments in their cytoplasm. 
      The cells proliferated rapidly, and the population doubling time was about 32 
      hours. The chromosome number showed a narrow distribution of diploid range. 
      Abnormal constitution was observed in all cells by G-band karyotyping. The 
      culture cells were easily transplanted into the subcutis of nude mouse and 
      produced the tumor resembling the original tumor.
FAU - Ishiwata, Isamu
AU  - Ishiwata I
AD  - Ishiwata Obstetrics and gynecology Hosp.
FAU - Ishiwata, Chieko
AU  - Ishiwata C
FAU - Ishiwata, Emiko
AU  - Ishiwata E
FAU - Iguchi, Megumi
AU  - Iguchi M
FAU - Sato, Yoshiro
AU  - Sato Y
FAU - Sonobe, Makoto
AU  - Sonobe M
FAU - Kiguchi, Kazushige
AU  - Kiguchi K
FAU - Tachibana, Toshiaki
AU  - Tachibana T
FAU - Ishikawa, Hiroshi
AU  - Ishikawa H
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Hum Cell
JT  - Human cell
JID - 8912329
SB  - IM
MH  - Aged
MH  - Animals
MH  - Brain Neoplasms/genetics/metabolism/*pathology/ultrastructure
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Female
MH  - Glioblastoma/genetics/metabolism/*pathology/ultrastructure
MH  - Humans
MH  - Karyotyping
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Nude
MH  - Microscopy, Electron, Transmission
MH  - Neoplasm Transplantation
EDAT- 2005/09/01 09:00
MHDA- 2005/11/08 09:00
CRDT- 2005/09/01 09:00
PHST- 2005/09/01 09:00 [pubmed]
PHST- 2005/11/08 09:00 [medline]
PHST- 2005/09/01 09:00 [entrez]
AID - 10.1111/j.1749-0774.2005.tb00058.x [doi]
PST - ppublish
SO  - Hum Cell. 2005 Mar;18(1):59-65. doi: 10.1111/j.1749-0774.2005.tb00058.x.